Infrafrontier

Generation of custom mouse and rat mutant strains using the latest genome-editing technologies by expert laboratories.

Custom genetic engineering and genome editing services to develop physiologically relevant mouse and rat models, ranging from strategy design to functional validation using CRISPR/Cas9 and ES cell technologies.

Model Generation Service that includes zygote electroporation of CRISPR/Cas9-based targeting tools, pronuclear microinjection of DNA constructs into mouse zygotes, microinjection of targeted ES cell lines into morulas, mouse archiving, and recovery of live mice from cryopreserved embryos and sperm.

Custom genetic engineering and genome editing service for generating precision cancer mouse and rat models using CRISPR-Cas9 technology, ranging from strategic design to functional validation.

Comprehensive genome engineering resource offering design, model production, and validation of custom mouse models using classical gene targeting, CRISPR/Cas9 genome editing, and additive transgenesis.

Service providing generation of genetically engineered mice based on zygote manipulation by CRISPR/Cas9 procedures, covering design, hands-on steps, breeding procedures, and long-term cryopreservation.

The UK’s national facility for mouse genetics and the use of mouse models for the preclinical study of human disease, offering archiving, breeding, phenotyping, and genome engineering services.

Advanced infrastructure and integrated technologies provided by CIPHE to support the development of therapies and vaccines targeting infectious and immune-mediated diseases, utilizing ANSM-accredited BSL-2 and BSL-3 facilities.

Services for the generation and development of genetically modified mouse models.

Generation of genetically edited mice with CRISPR/Cas9 tools via electroporation, offered by the Mouse Embryo Cryopreservation Facility at the National Centre for Biotechnology (CNB-CSIC).

Comprehensive preclinical phenotyping services for mouse and rat models encompassing neurobiology and behaviour assessments, cardiovascular system evaluations, clinical chemistry, hematology profiling, metabolic exploration, histopathology, embryology, and oncology studies.

CRISPR/Cas9 technology service enabling the modification of the genome to produce precise and unique knock-out and knock-in GM mouse models.

Advanced BioSafety Level 3 (BSL-3) services offering scientists access to high-containment rodent model studies for testing therapeutics against infectious diseases.

Comprehensive collection of tools and know-how for systematic and deep-drilling behavioural, physiological and morphological assessment of mice and rats models in a controlled specific pathogen-free environment.

The Centre for Phenogenomics (TCP) produces and phenotypes mouse models for discovery and biomedical research across a range of body systems for research, preclinical assessment, and drug target validation.

Germ-free derivation of user-provided mouse strains used for different gnotobiotic studies shedding light on gut microbiota.

Selected strain collection focused on genome editing mice for medical research applications.

French National Infrastructure unit dedicated to mouse and rat models, providing zootechny, imaging, distribution, archiving, and SOPF or germ-free mouse models.

An INFRAPLUS service aimed at expanding the characterisation of mouse models before their use, verifying the integrity of genetic alteration and genetic background in a genetically altered line.

The Axenic Service includes axenisation of mouse strains starting from live animals, frozen embryos or sperm, and the exportation of germ-free mice within purpose-made shippers. The facility also supplies organs, tissues, and biofluids harvested from germ-free mice, alongside extended breeding and gnotobiotic services.

Typing and Archiving of Animal Models (TAAM) is an academic unit providing mouse and rat model services including breeding, archiving, distribution, health monitoring, in-vivo imaging, gnotobiotic models, and cryopreservation.

Mutant mouse models generated by researchers around the world are archived using the latest cryopreservation techniques in the European Mouse Mutant Archive (EMMA).

Biomedical research organization and animal facility maintaining various animal species, featuring a fully equipped germ-free facility, experimental gnotobiology laboratory, and reproductive biology services.

Cryopreservation services for archiving mouse lines and genetic resources.

The Typage et Archivage d’Animaux Modèles (TAAM) at CNRS offers breeding isolators and capacity to provide experimental batches of germ-free or gnotobiotic, inbred or genetically modified mice.

Physiological assessment of mice or rats with more than 700 parameters per model in basic phenotyping, expanded to disease-focused special screens or customised pipelines.

Specialised phenotyping services provided via the German Mouse Clinic (GMC).

Microinjection of a transgene construct into zygotes where foreign DNA is integrated into the genome in a random manner.

Large-scale, standardised, and comprehensive phenotypic analysis as well as customised phenotyping of mouse mutants from various sources provided by the German Mouse Clinic (GMC) at Helmholtz Munich.

Custom service to convert knockout-first alleles into alternative desired alleles.

Preclinical evaluation services for translational research and functional genomics.

Access unit covering the derivation of a single mouse mutant line into a germ-free condition from a breeding nucleus or frozen materials.

The deposited mutant mouse lines are sent to researchers around the globe as frozen gametes or live cohorts.

Mouse embryonic stem cells cultured in pluripotent stage with mutations targeted to a specific locus to produce knock-out and knock-in GM mouse models.

Microinjection of correctly targeted ES cells into morula-stage mouse embryos to produce chimeric mice.

Specialized facility providing production of germ-free mice by caesarian section, husbandry, quality controls, and production of gnotoxenic mice.

Rederivation service to generate live heterozygous animals from cryopreserved stocks.

Intravital microscopy of mouse tumors labelled with fluorescent markers in combination with label free second and third harmonic generation imaging in the brain and skin, providing imaging windows implanted on the skull or in the skin.

Archiving and distribution node for mouse models via EMMA.

Advanced imaging analysis services for biological and cellular characterization.

Comprehensive first line phenotyping service of a mutant line with appropriate control, provided as a Transnational Access activity under the INFRAFRONTIER-I3 project.

A standardized infection pipeline and trans-national access service provided by INFRAFRONTIER and CIPHE to assess novel potential COVID-19 vaccines and treatment compounds in preclinical mouse models.

Tamoxifen-inducible Cre recombinase specific of renal tubular epithelia.

Service offering tissue processing, embedding, sectioning, staining, and quality-controlled digitization of whole-slide histological images.

Service to assess mRNA expression levels to ensure that the mutated allele exhibits the expected functionality.

Core animal facility in Oeiras, Portugal offering germ-free and strict SPF mouse models, housing about 50,000 mice covering close to 100 different inbred strains.

An INFRAPLUS service aimed at the generation of mutant embryonic fibroblasts and mouse embryonic stem cells to support scientists in developing mutant cell models.

Tamoxifen-inducible Cre deleter specific of vascular endothelial cells.

This service combines mouse breeding and experimental animal pathology, including genotyping, bi-weekly tumor palpation, full necropsy, and comprehensive histopathology reports.

Mouse strain archived by EMMA (European Mouse Mutant Archive), available as frozen sperm or via rederivation.

The European Mouse Mutant Archive is a non-profit repository for the collection, archiving via cryopreservation, and distribution of relevant mutant mouse and rat strains essential for basic biomedical research.

A unique archive for the distribution of GEMM-ESC lines created as part of the EMMA project.

Assisted reproduction and rederivation services including embryo transfer in laboratory mice.

Selected strain collection providing access to knockout mouse resources.

Archives and distributes mouse strains developed inhouse or submitted by external research partners as part of the European Mouse Mutant Archive.

The Austrian node of the European Mouse Mutant Archive offering archiving, distribution, and repository services.

Cryopreserved mouse embryos distributed by EMMA for strain acquisition and research use.

Cryopreserved mouse sperm provided in straws or vials for IVF and rederivation.

Services related to the Italian node of the European Mouse Mutant Archive (EMMA) hosted by the Consiglio Nazionale delle Ricerche (CNR).

Serves as the northernmost INFRAFRONTIER/EMMA node holding more than 360 mouse lines for distribution to scientists worldwide.

French node of the European Mouse Mutant Archive provided by PHENOMIN-ICS.

Genotypic and phenotypic data curation and disease model annotation services.

A nomenclature service provided by EMMA for archived mice according to the rules and guidelines established by the International Committee on Standardized Genetic Nomenclature for Mice.

Selected strain collection utilizing Mosaic Analysis with Double Markers for genetic studies.

Provides services for tissue processing and phenotyping with slide scanning and access to advanced image analysis.