Validogen
VALIDOGEN is a leading contract research & development organization specializing in recombinant protein expression using its exclusive UNLOCK PICHIA® technology. With over 15 years of expertise, the company provides innovative solutions for successful and economically viable protein production in Pichia pastoris, serving industries such as biopharma, food & feed, industrial biotechnology, and diagnostics. Their services include strain and process development, feasibility studies, and technology transfer, supported by a broad portfolio of proprietary tools and strategies to maximize protein yields and quality.
Industries
Nr. of Employees
small (1-50)
Validogen
Parkring 18, 8074 Raaba-Grambach, Austria
Patents
Products
Proprietary Pichia expression toolbox
A documented set of expression tools including promoter variants, platform strains, secretion signals, helper factors and glycoengineering components for optimizing Pichia-based protein production.
Technical downloads: white papers and case studies
Published technical white papers, case studies and flyers describing methanol-free processes, case data (e.g., VHH production) and technology overviews for Pichia-based protein expression.
Proprietary Pichia expression toolbox
A documented set of expression tools including promoter variants, platform strains, secretion signals, helper factors and glycoengineering components for optimizing Pichia-based protein production.
Technical downloads: white papers and case studies
Published technical white papers, case studies and flyers describing methanol-free processes, case data (e.g., VHH production) and technology overviews for Pichia-based protein expression.
Services
Rapid assessment of expression potential in Pichia using cloning, transformation, microscale screening and small-scale cultivations, with delivery of non-GMP protein samples and feasibility reports.
Combinatorial strain engineering and screening to produce high-performance Pichia expression strains, including ARMs-free and auxotrophic strain options, and delivery of characterized cell banks.
Development and optimization of fermentation protocols (including methanol-free processes) and downstream purification processes, scalable from bench to industrial volumes with full documentation for transfer.
Analytical testing of product and process intermediates using electrophoretic, chromatographic, immunoassay and impurity quantification methods to support process development and documentation.
Preparation of glycerol cell banks and molecular characterization services including purity/viability checks, integration assessment, whole genome sequencing, and copy number determination.
Support for transfer of strains, processes and documentation to customer sites or CMOs and assistance in scaling cultivation processes to industrial volumes.
Rapid assessment of expression potential in Pichia using cloning, transformation, microscale screening and small-scale cultivations, with delivery of non-GMP protein samples and feasibility reports.
Combinatorial strain engineering and screening to produce high-performance Pichia expression strains, including ARMs-free and auxotrophic strain options, and delivery of characterized cell banks.
Development and optimization of fermentation protocols (including methanol-free processes) and downstream purification processes, scalable from bench to industrial volumes with full documentation for transfer.
Analytical testing of product and process intermediates using electrophoretic, chromatographic, immunoassay and impurity quantification methods to support process development and documentation.
Preparation of glycerol cell banks and molecular characterization services including purity/viability checks, integration assessment, whole genome sequencing, and copy number determination.
Support for transfer of strains, processes and documentation to customer sites or CMOs and assistance in scaling cultivation processes to industrial volumes.
Expertise Areas
- Recombinant protein expression in yeast
- Pichia pastoris strain engineering
- Promoter and secretion signal optimization
- High-throughput screening and microbioreactor workflows
Key Technologies
- Pichia pastoris expression systems
- AOX1-based promoter variants (inducible and methanol-free)
- Secretion signal engineering
- Helper factor co-expression (chaperones, TFs, membrane proteins)
News & Updates
Expansion of proprietary secretion signals beyond α-mating factor to overcome secretion bottlenecks and boost recombinant protein expression.
VALIDOGEN representatives will attend BIO-Europe Spring 2025 to showcase their protein production process development services.
European Biotechnology Magazine features an article on VALIDOGEN's UNLOCK PICHIA® system, highlighting its potential for industry applications.
An article discussing recombinant protein production in Pichia using VALIDOGEN's technology, emphasizing high yields and safety.
Expansion of proprietary secretion signals beyond α-mating factor to overcome secretion bottlenecks and boost recombinant protein expression.
VALIDOGEN representatives will attend BIO-Europe Spring 2025 to showcase their protein production process development services.
European Biotechnology Magazine features an article on VALIDOGEN's UNLOCK PICHIA® system, highlighting its potential for industry applications.
An article discussing recombinant protein production in Pichia using VALIDOGEN's technology, emphasizing high yields and safety.