Validogen
VALIDOGEN is a leading contract research & development organization specializing in recombinant protein expression using its exclusive UNLOCK PICHIA® technology. With over 15 years of expertise, the company provides innovative solutions for successful and economically viable protein production in Pichia pastoris, serving industries such as biopharma, food & feed, industrial biotechnology, and diagnostics. Their services include strain and process development, feasibility studies, and technology transfer, supported by a broad portfolio of proprietary tools and strategies to maximize protein yields and quality.
Industries
Nr. of Employees
small (1-50)
Validogen
Raaba, Steiermark, Austria, Europe
Products
Proprietary Pichia expression toolbox
A documented set of expression tools including promoter variants, platform strains, secretion signals, helper factors and glycoengineering components for optimizing Pichia-based protein production.
Technical downloads: white papers and case studies
Published technical white papers, case studies and flyers describing methanol-free processes, case data (e.g., VHH production) and technology overviews for Pichia-based protein expression.
Proprietary Pichia expression toolbox
A documented set of expression tools including promoter variants, platform strains, secretion signals, helper factors and glycoengineering components for optimizing Pichia-based protein production.
Technical downloads: white papers and case studies
Published technical white papers, case studies and flyers describing methanol-free processes, case data (e.g., VHH production) and technology overviews for Pichia-based protein expression.
Services
Expression Feasibility Studies
Rapid assessment of expression potential in Pichia using cloning, transformation, microscale screening and small-scale cultivations, with delivery of non-GMP protein samples and feasibility reports.
Pichia Strain Development
Combinatorial strain engineering and screening to produce high-performance Pichia expression strains, including ARMs-free and auxotrophic strain options, and delivery of characterized cell banks.
Bioprocess Development (Fermentation & Purification)
Development and optimization of fermentation protocols (including methanol-free processes) and downstream purification processes, scalable from bench to industrial volumes with full documentation for transfer.
Analytical and Characterization Services
Analytical testing of product and process intermediates using electrophoretic, chromatographic, immunoassay and impurity quantification methods to support process development and documentation.
Cell Banking and Line Characterization
Preparation of glycerol cell banks and molecular characterization services including purity/viability checks, integration assessment, whole genome sequencing, and copy number determination.
Technology Transfer and Scale-Up Support
Support for transfer of strains, processes and documentation to customer sites or CMOs and assistance in scaling cultivation processes to industrial volumes.
Expression Feasibility Studies
Rapid assessment of expression potential in Pichia using cloning, transformation, microscale screening and small-scale cultivations, with delivery of non-GMP protein samples and feasibility reports.
Pichia Strain Development
Combinatorial strain engineering and screening to produce high-performance Pichia expression strains, including ARMs-free and auxotrophic strain options, and delivery of characterized cell banks.
Bioprocess Development (Fermentation & Purification)
Development and optimization of fermentation protocols (including methanol-free processes) and downstream purification processes, scalable from bench to industrial volumes with full documentation for transfer.
Analytical and Characterization Services
Analytical testing of product and process intermediates using electrophoretic, chromatographic, immunoassay and impurity quantification methods to support process development and documentation.
Cell Banking and Line Characterization
Preparation of glycerol cell banks and molecular characterization services including purity/viability checks, integration assessment, whole genome sequencing, and copy number determination.
Technology Transfer and Scale-Up Support
Support for transfer of strains, processes and documentation to customer sites or CMOs and assistance in scaling cultivation processes to industrial volumes.
Expertise Areas
- Recombinant protein expression in yeast
- Pichia pastoris strain engineering
- Promoter and secretion signal optimization
- High-throughput screening and microbioreactor workflows
Key Technologies
- Pichia pastoris expression systems
- AOX1-based promoter variants (inducible and methanol-free)
- Secretion signal engineering
- Helper factor co-expression (chaperones, TFs, membrane proteins)