Mtibio
mtibio is a company dedicated to providing high-quality, customizable protein expression and purification services across multiple platforms including E. coli, baculovirus, and mammalian cells. Founded in 2008, it aims to streamline protein production with a focus on superior quality, responsiveness, and cost-effectiveness. The company offers proprietary ultra-low endotoxin purification and endotoxin removal services, serving clients in biotechnology, agriculture, pharmaceutical sectors, and academia. Its mission is to enable clients to meet and surpass their research goals through personalized service and competitive pricing.
Industries
Nr. of Employees
small (1-50)
Mtibio
Services
E. coli protein expression service
Contract expression of recombinant proteins in E. coli including gene construct assembly, transformation, expression testing and scale-up to liter-scale production.
Baculovirus/insect cell expression service
Generation and amplification of recombinant baculovirus, expression testing in insect cell lines and scale-up for production of secreted and intracellular proteins.
Transient mammalian cell expression service
Transient transfection workflows in HEK293 and CHO cells including construct design, transfection-grade DNA production, expression testing and scaled production runs.
Custom gene synthesis and construct assembly
De novo gene synthesis with codon optimization for the selected expression system, subcloning into expression vectors and delivery of sequence-verified constructs and trace files.
cDNA cloning and sequencing
Acquisition of tissues, mRNA isolation, RT-PCR quality control using housekeeping primers and cDNA cloning/sequencing including work for non-annotated species.
One-step protein purification
Lysate preparation and single-step chromatographic capture for His-tagged, GST-fusion and ion-exchange workflows with monitoring and fraction analysis.
E. coli protein expression service
Contract expression of recombinant proteins in E. coli including gene construct assembly, transformation, expression testing and scale-up to liter-scale production.
Baculovirus/insect cell expression service
Generation and amplification of recombinant baculovirus, expression testing in insect cell lines and scale-up for production of secreted and intracellular proteins.
Transient mammalian cell expression service
Transient transfection workflows in HEK293 and CHO cells including construct design, transfection-grade DNA production, expression testing and scaled production runs.
Custom gene synthesis and construct assembly
De novo gene synthesis with codon optimization for the selected expression system, subcloning into expression vectors and delivery of sequence-verified constructs and trace files.
cDNA cloning and sequencing
Acquisition of tissues, mRNA isolation, RT-PCR quality control using housekeeping primers and cDNA cloning/sequencing including work for non-annotated species.
One-step protein purification
Lysate preparation and single-step chromatographic capture for His-tagged, GST-fusion and ion-exchange workflows with monitoring and fraction analysis.
Expertise Areas
- Recombinant protein expression (E. coli, baculovirus/insect, mammalian transient)
- Protein purification and chromatographic methods
- Endotoxin control and removal for biologics
- Gene synthesis and construct design
Key Technologies
- E. coli recombinant expression
- Baculovirus expression systems
- Transient mammalian transfection (HEK293, CHO)
- Codon optimization and gene synthesis