Portable field-deployable nucleic acid sequencing kit
Inventors
MITCHELL, Shane • OMARI, Masooda • Thomas, Sterling • Thompson, Leo
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Assignees
Member
NoblisNoblis is a nonprofit research and technical organization supporting federal missions in defense, health, environment, and security. Emphasizing applied sciences, engineering, digital transformation, artificial intelligence, cloud, and cybersecurity, Noblis provides objective solutions for government agencies confronting complex operational and scientific challenges.
Noblis is a nonprofit research and technical organization supporting federal missions in defense, health, environment, and security. Emphasizing applied sciences, engineering, digital transformation, artificial intelligence, cloud, and cybersecurity, Noblis provides objective solutions for government agencies confronting complex operational and scientific challenges.
Publication Number
US-12195729-B2
Publication Date
2025-01-14
Expiration Date
Abstract
A portable kit for nucleic acid sequencing is disclosed, the kit including a DNA extraction system configured to perform a DNA extraction protocol, a DNA sequencing preparation system configured to perform a DNA sequencing preparation protocol, a sequencer system, and a portable enclosure configured to house the DNA extraction system, the sequencer preparation system, and the sequencer system.
Core Innovation
The disclosed invention provides a portable kit for sequencing nucleic acid from a sample. The portable kit includes a DNA extraction system configured to extract DNA using a DNA extraction protocol and a DNA sequencing preparation system configured to prepare the extracted DNA using a DNA sequencing preparation protocol. The kit further includes a sequencer system having a sequencer device, a heating device, and an insulated casing, and a portable enclosure enclosing the DNA extraction system, the DNA sequencing preparation system, and the sequencer system.
The sequencer device is surrounded by air, the heating device is external to the sequencer device, and the insulated casing and the heating device are configured to maintain an operational temperature of the sequencer device when the temperature of the air surrounding the sequencer device is less than or equal to about 2° C. Cold-air operation is addressed by enclosing the sequencer device and applying external heating through the insulated casing and heating device.
The invention further includes field sequencing workflows that integrate extracting, quantifying, preparation, and sequencing within the portable enclosure. A method is disclosed in which DNA is extracted using the DNA extraction system and centrifuges, the extracted DNA is quantified using a fluorometer, and the extracted DNA is prepared for sequencing using centrifuges, a mixer, the DNA sequencing preparation system, and a sample preparation device.
Claims Coverage
The document includes three independent claims. Across these claims, the inventive features center on a portable enclosure, a sequencer device surrounded by air, external heating and an insulated casing for maintaining operational temperature when the air is less than or equal to about 2° C., and integration of DNA extraction, DNA sequencing preparation, quantifying, sequencing, and centrifuge ramping behavior in a portable field workflow.
Portable nucleic-acid sequencing kit with temperature-maintaining sequencer casing
A portable kit for sequencing nucleic acid from a sample comprising a DNA extraction system, a DNA sequencing preparation system, a sequencer system including a sequencer device, a heating device, and an insulated casing, and a portable enclosure enclosing the systems, wherein the insulated casing and the heating device are configured to maintain an operational temperature of the sequencer device when the air surrounding the sequencer device is less than or equal to about 2° C.
Portable field method for extracting, quantifying, preparing, and sequencing with cold-operating enclosure
A method of sequencing DNA from a sample using a portable field kit comprising a DNA extraction system, a DNA sequencing preparation system, a sample preparation device, a first centrifuge, a second centrifuge, a mixer, a fluorometer, and a sequencer system with a heating device and an insulated casing, the method comprising extracting DNA, quantifying the extracted DNA using the fluorometer, preparing the extracted DNA for sequencing, and sequencing the prepared extracted using the sequencer system.
Centrifuge ramping method in portable kit with intermediate-speed hold
A method of ramping a plurality of centrifuges using a portable kit, comprising ramping the first centrifuge to an intermediate speed, operating the first centrifuge at the intermediate speed for a predetermined amount of time without increasing its speed above the intermediate speed, and after that further ramping the first centrifuge from the intermediate speed to a target speed.
The independent claims consistently require a portable enclosure and a sequencer system with external heating and an insulated casing for low-temperature operation, while the field workflow integrates DNA extraction, DNA sequencing preparation, centrifuges, mixing, quantifying with a fluorometer, and sequencing. A separate independent claim specifies an intermediate-speed hold before further centrifuge ramping.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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