Functional ligands to colistin
Inventors
Batchelor, Robert • Jackson, George W. • Nguyen, Truong • Sankarapani, Vinoth
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Assignees
Member
Base Pair BiotechnologiesBase Pair Biotechnologies specializes in custom aptamer discovery and development for research, diagnostics, therapeutics, and industrial applications. The company leverages proprietary multiplex selection, advanced bioinformatics, and chemical modification techniques to develop high-affinity and selective nucleic acid aptamers. Base Pair enables affinity reagent development, biosensor design, and molecular detection for a broad range of targets and partners across academia and industry.
Base Pair Biotechnologies specializes in custom aptamer discovery and development for research, diagnostics, therapeutics, and industrial applications. The company leverages proprietary multiplex selection, advanced bioinformatics, and chemical modification techniques to develop high-affinity and selective nucleic acid aptamers. Base Pair enables affinity reagent development, biosensor design, and molecular detection for a broad range of targets and partners across academia and industry.
Publication Number
US-11608356-B1
Publication Date
2023-03-21
Expiration Date
Abstract
The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as antibiotics, such as colistin.
Core Innovation
The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as antibiotics, such as colistin.
The background describes that aptamers are commonly identified by an in vitro method of selection sometimes referred to as Systematic Evolution of Ligands by EXponential enrichment or “SELEX”, which typically begins with a very large pool of randomized polynucleotides and is generally narrowed to one aptamer ligand per molecular target. In one exemplary aspect of the invention, generation of functional biomolecules may be performed against more than one or multiple targets simultaneously within a single system, such as the generation of functional nucleic acid ligands within a single reaction volume, enabling high capacity, multiplexed identification procedures and the matching of binding members to particular targets.
Claims Coverage
The patent contains one independent claim. The extracted inventive features count: 1.
Artificial ligand binding to colistin
An artificial ligand binding to colistin comprising a non-naturally occurring nucleic acid sequence having 100% sequence identity to a sequence selected from the group consisting of SEQ ID Nos. 1-100.
The independent claim covers an artificial ligand that binds colistin, defined as a non-naturally occurring nucleic acid sequence identical to one of SEQ ID Nos. 1-100.
Stated Advantages
Detection, quantification, and/or other diagnostic applications for detecting or quantifying levels of antibiotics such as colistin in body fluids or tissues or samples thereof.
Monitoring dosage of an antibiotic in a patient.
High-capacity, multiplexed identification procedures that may save time, expense, and physical space compared to single target identification processes.
Ability to identify and/or eliminate biomolecules that bind or have a tendency to bind to multiple targets.
Documented Applications
Detection and quantification of colistin for diagnostic applications, including monitoring dosage of an antibiotic in a patient.
Use in electrochemical sensors, gold nanoparticle assays, enzyme linked aptamer sorbent assays (ELASA), pull down assays (immunoprecipitation), and microplate/well assays.
Creation of aptamer beacons (aptabeacons) that produce a detectable signal upon binding to a target molecule.
Simultaneous generation of numerous different functional nucleic acids against multiple target molecules within a single reaction volume or on target arrays (multiplexed SELEX).
Application to target sets including arrays and histology sections as targets.
Use with peptide display and nucleic acid-linked peptide selection methods, including phage display, mRNA display, and ribosome display, for identifying peptide binders and linking them to nucleic acid identifiers.
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