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Assignees
Member
Base Pair BiotechnologiesBase Pair Biotechnologies specializes in custom aptamer discovery and development for research, diagnostics, therapeutics, and industrial applications. The company leverages proprietary multiplex selection, advanced bioinformatics, and chemical modification techniques to develop high-affinity and selective nucleic acid aptamers. Base Pair enables affinity reagent development, biosensor design, and molecular detection for a broad range of targets and partners across academia and industry.
Base Pair Biotechnologies specializes in custom aptamer discovery and development for research, diagnostics, therapeutics, and industrial applications. The company leverages proprietary multiplex selection, advanced bioinformatics, and chemical modification techniques to develop high-affinity and selective nucleic acid aptamers. Base Pair enables affinity reagent development, biosensor design, and molecular detection for a broad range of targets and partners across academia and industry.
Publication Number
US-10538770-B2
Publication Date
2020-01-21
Expiration Date
Abstract
The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules such as chikungunya viral proteins, such as chikungunya envelope protein E1.
Core Innovation
The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules such as chikungunya viral proteins, such as chikungunya envelope protein E1.
A method for generating functional biomolecules includes obtaining a library of biomolecules, contacting the library with more than one target within a single system, partitioning non-binding members, amplifying binding members, and marking or tagging binding members so they may be matched to particular targets. Embodiments include affixing a plurality of target molecules to a substrate in clusters or "spots," applying a library to the array, tagging bound library members with identifiers associated with spot locations, and utilizing multiplexed identification procedures to save time, expense and physical space and to identify or eliminate biomolecules that bind multiple targets; embodiments utilizing SELEX are described [procedural detail omitted for safety].
Claims Coverage
Two inventive features are identified from the independent claims.
Short non-naturally occurring nucleic acid ligand with greater than 80% sequence identity to SEQ IDs 1-56
An artificial ligand binding to chikungunya viral envelope protein E1 comprising a non-naturally occurring nucleic acid sequence of under 40 nucleotides in length having substantial homology and greater than 80% sequence identity to a sequence selected from the group consisting of SEQ IDs 1-56.
Non-naturally occurring nucleic acid ligand with 100% sequence identity to SEQ IDs 1-56
An artificial ligand binding to chikungunya viral envelope protein E1 comprising a non-naturally occurring nucleic acid sequence having 100% sequence identity to a sequence selected from the group consisting of SEQ ID Nos. 1-56.
The independent claims cover artificial, non-naturally occurring nucleic acid ligands that bind chikungunya envelope protein E1, specified either as sequences under 40 nucleotides with >80% identity to SEQ IDs 1-56 or as sequences with 100% identity to SEQ IDs 1-56.
Stated Advantages
May be utilized for detection, quantification, and other diagnostic applications.
May be utilized in therapeutic manners, including binding to chikungunya viral particles to neutralize effects.
High capacity, multiplexed identification procedures may save time, expense, and physical space compared to single target identification processes.
Method may be utilized to identify and/or eliminate biomolecules that bind or have a tendency to bind to multiple targets.
Documented Applications
Use as sensors and therapeutic tools, including regulation of cellular processes and guiding drugs to specific cellular targets.
Immobilized capture elements in a microarray format for identifying binding members of a biomolecule library.
Detection and quantification of chikungunya virus in subjects, samples from subjects, and in carriers such as mosquitos.
Therapeutic binding to chikungunya viral particles to neutralize effects.
Aptamer-based molecular beacons (aptabeacons) that produce a detectable signal upon aptamer binding for detection of target molecules.
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