Vaccine R & D Center
Academic vaccine research center focused on proteome-scale antigen discovery, serology-based biomarker identification, and translational vaccine formulation and preclinical testing. Core competencies include high-throughput cloning and expression, construction and use of high-density protein microarrays and other array formats, bioinformatics/biostatistics for antigen classification and antigenicity prediction, adjuvant chemistry and nanoparticle-based delivery formulation, and preclinical immunobiology with access to BSL-3 animal facilities. The center operates core services and offers hands-on training in array technologies and data analysis.
Industries
Nr. of Employees
small (1-50)
Vaccine R & D Center
Philip L. Felgner, Ph.D., Department of Physiology & Biophysics, University of California, Irvine, 3052 Hewitt Hall, Irvine, CA 92697-4560
Products
Expression-ready ORF/plasmid libraries
Extensive cloned ORF collections and plasmid libraries spanning many microorganisms, maintained for expression and array printing to support antigen discovery and assay development.
Custom printed microarray slides
Customizable printed microarray slides configured for multiple subarrays per slide and intended for high-throughput serological profiling and diagnostic evaluation.
Expression-ready ORF/plasmid libraries
Extensive cloned ORF collections and plasmid libraries spanning many microorganisms, maintained for expression and array printing to support antigen discovery and assay development.
Custom printed microarray slides
Customizable printed microarray slides configured for multiple subarrays per slide and intended for high-throughput serological profiling and diagnostic evaluation.
Services
End-to-end microarray services including high-throughput cloning and ORF library access, expression workflows (including cell-free), automated printing of customizable slides, serology probing and quantitative data analysis.
Instructor-led workshops covering array types and substrates, high-throughput cloning and expression, printing technologies, probing/detection methods, quantification, normalization and initial data analysis.
End-to-end microarray services including high-throughput cloning and ORF library access, expression workflows (including cell-free), automated printing of customizable slides, serology probing and quantitative data analysis.
Instructor-led workshops covering array types and substrates, high-throughput cloning and expression, printing technologies, probing/detection methods, quantification, normalization and initial data analysis.
Expertise Areas
- Proteome-scale antigen discovery
- Protein and peptide microarray assay development
- High-throughput cloning and ORFeome management
- Cell-free expression systems for antigen production
Key Technologies
- Protein microarrays
- Peptide arrays and reverse-phase protein arrays (RPPA)
- High-throughput cloning workflows (ORFeome construction)
- Cell-free in vitro transcription–translation
News & Updates
Coverage noting the center director among laureates for technical and scientific research (2021).
Preprints describing coronavirus antigen microarray analyses and serologic cross-reactivity and antibody profiling for SARS-CoV-2 (2020).
Published proteome microarray studies profiling humoral responses to vaccinia and identifying major neutralizing targets.
Study of proteome-scale antibody responses and outcomes of Mycobacterium tuberculosis infection in nonhuman primates and human patients.
Construction and use of an extensive ORF collection (tens of thousands of genes) and application to probe >25,000 sera for antigen discovery and serodiagnostics.
Coverage noting the center director among laureates for technical and scientific research (2021).
Preprints describing coronavirus antigen microarray analyses and serologic cross-reactivity and antibody profiling for SARS-CoV-2 (2020).
Published proteome microarray studies profiling humoral responses to vaccinia and identifying major neutralizing targets.
Study of proteome-scale antibody responses and outcomes of Mycobacterium tuberculosis infection in nonhuman primates and human patients.
Construction and use of an extensive ORF collection (tens of thousands of genes) and application to probe >25,000 sera for antigen discovery and serodiagnostics.