NanoTag Biotechnologies
Provider of single-domain antibody (sdAb) discovery, engineered sdAb reagents, affinity selector resins and custom protein services for research and preclinical applications. Offers animal immunization and library-based discovery workflows, affinity-based B-cell selection, site-specific fluorophore conjugation with defined stoichiometry, fixation-enhancing tags for microscopy, recombinant sdAb–Fc fusions, bispecific sdAb constructs, selector resins for tag-based purification, and CRO-style expression, purification and conjugation services. Publishes protocols, manuals and safety data sheets for products and documents licensing restrictions for certain materials.
Industries
Nr. of Employees
small (1-50)
NanoTag Biotechnologies
Products
Fluorophore-conjugated single-domain antibody reagents (single- and multi-dye)
Fluorescently labeled sdAb reagents with defined numbers of fluorophores at known positions available in single-fluorophore (quantitative) and multi-fluorophore formats for high-sensitivity imaging and super-resolution applications.
Affinity selector resins for tagged proteins (agarose and magnetic)
Covalently immobilized sdAbs on cross-linked agarose or magnetic beads for capture and purification of tagged proteins and fusions; oriented attachment reduces non-specific binding and preserves functionality.
Recombinant sdAb–Fc fusion (heavy-chain) reagents
Genetic fusions of sdAbs to mammalian Fc domains produced in HEK cells to provide divalent reagents compatible with Fc-based detection and amplification systems.
Peptide elution reagent for tag-based affinity purification
Lyophilized peptide supplied with instructions for reconstitution to prepare native elution buffers for peptide-elutable selector resins; storage and handling guidance provided.
Mini centrifuge columns for small-volume resin handling
Disposable mini columns with internal filter for processing small volumes of bead-based resins in standard microcentrifuges.
Multiplexing blocker reagents
Protein mixtures containing sdAbs that bind common immunoglobulins to block cross-reactivity and enable multiplexed detection workflows.
Fluorophore-conjugated single-domain antibody reagents (single- and multi-dye)
Fluorescently labeled sdAb reagents with defined numbers of fluorophores at known positions available in single-fluorophore (quantitative) and multi-fluorophore formats for high-sensitivity imaging and super-resolution applications.
Affinity selector resins for tagged proteins (agarose and magnetic)
Covalently immobilized sdAbs on cross-linked agarose or magnetic beads for capture and purification of tagged proteins and fusions; oriented attachment reduces non-specific binding and preserves functionality.
Recombinant sdAb–Fc fusion (heavy-chain) reagents
Genetic fusions of sdAbs to mammalian Fc domains produced in HEK cells to provide divalent reagents compatible with Fc-based detection and amplification systems.
Peptide elution reagent for tag-based affinity purification
Lyophilized peptide supplied with instructions for reconstitution to prepare native elution buffers for peptide-elutable selector resins; storage and handling guidance provided.
Mini centrifuge columns for small-volume resin handling
Disposable mini columns with internal filter for processing small volumes of bead-based resins in standard microcentrifuges.
Multiplexing blocker reagents
Protein mixtures containing sdAbs that bind common immunoglobulins to block cross-reactivity and enable multiplexed detection workflows.
Services
sdAb discovery and screening services (immunization and library workflows)
Custom discovery projects including antigen design, immunization, PBMC-derived or synthetic library construction, tailored panning strategies, candidate sequencing and initial in vitro validation; delivery options range from nucleic acid/blood material to purified clones.
Custom protein expression, purification and process development
Expression and purification in bacterial and mammalian systems with services including codon optimization, construct design, clone screening, QC, scale-up, lyophilization, endotoxin removal and options for animal-free media.
Custom conjugation and reagent labeling services
Site-specific conjugation of sdAbs to fluorescent dyes and other labels using thiol-reactive chemistries, with control of labeling stoichiometry and compatibility with a broad range of dyes.
sdAb discovery and screening services (immunization and library workflows)
Custom discovery projects including antigen design, immunization, PBMC-derived or synthetic library construction, tailored panning strategies, candidate sequencing and initial in vitro validation; delivery options range from nucleic acid/blood material to purified clones.
Custom protein expression, purification and process development
Expression and purification in bacterial and mammalian systems with services including codon optimization, construct design, clone screening, QC, scale-up, lyophilization, endotoxin removal and options for animal-free media.
Custom conjugation and reagent labeling services
Site-specific conjugation of sdAbs to fluorescent dyes and other labels using thiol-reactive chemistries, with control of labeling stoichiometry and compatibility with a broad range of dyes.
Expertise Areas
- Single-domain antibody discovery and screening
- Library-based lead identification (phage and ribosome display)
- Affinity-based B-cell selection from PBMCs
- Site-specific fluorescent labeling for microscopy
Key Technologies
- Single-domain antibodies (sdAbs / nanobodies)
- Phage display
- Ribosome display
- Site-specific fluorophore conjugation with controlled stoichiometry