NanoTag Biotechnologies

A unique, patented technology based on three single-domain antibodies recognizing a rationally designed epitope tag of only 13 amino acids, enabling a wide range of applications including high-resolution imaging, immobilization, affinity purification, and therapeutic applications.

High-affinity resin for efficient and clean purification of proteins fused to NbALFA (sdAb).

Innovative, high-affinity resin (Agarose & Magnetic) designed for efficient purification.

An innovative affinity resin designed for the highly efficient purification of ALFA-tagged proteins at low temperatures in physiological buffers. Supplied with 10 mg of ALFA elution peptide for competitive elution.

Innovative affinity resin (Agarose & Magnetic) designed for highly efficient purification with peptide elution capability.

sdAb anti-Human IgG (clone 2F3) is a single-domain antibody (nanobody, VHH) with high specificity for the constant Fc region of Human IgG isotypes, specifically recognizing Human IgG1, IgG3, and IgG4. It is part of the Smart Secondaries® product line and is used as a secondary reagent for immunolabeling and imaging applications.

A species-specific single-domain antibody targeting mouse IgG1, fused with Halo technology.

sdAb anti-Human IgG (clone 2E5) is an effective sdAb-based secondary reagent from the Smart Secondaries® product line, specifically targeting Human IgG.

sdAb anti-Mouse IgG2a/b (Smart Secondaries®) is a single-domain antibody (sdAb) that binds specifically and with high affinity to mouse IgG2a and IgG2b subtypes. It is used as a secondary tool for demanding immunolabeling and imaging applications.

A Halo-tagged version of a single-domain antibody recognizing the luminal/extracellular domain of Synaptotagmin 1 (Syt1).

Single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. Available conjugated to Biotin, DBCO, C-terminal Cysteine, or customized upon request.

A single-domain antibody (sdAb) that specifically and strongly binds to the CD45 protein.

A single-domain antibody (sdAb) that binds the Fc region of any rabbit IgG specifically and with high affinity, ideal for demanding immunolabeling and imaging applications.

FluoTag®-X2 anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. It features two fluorophores per sdAb molecule (X2).

FluoTag®-X2 anti-ALFA conjugated with Janelia Fluor® 635b for fluorescent detection of ALFA-tagged proteins.

FluoTag®-X2 anti-ALFA conjugated with LI-COR IRDye 800CW for fluorescent detection of ALFA-tagged proteins.

FluoTag®-X2 anti-ALFA conjugated with LI-COR IRDye 680RD for fluorescent detection of ALFA-tagged proteins.

FluoTag®-X2 anti-Mouse IgG1 (Smart Secondaries®) is a species-specific FluoTag® directed against IgG1 from Mouse, serving as a secondary reagent.

A Smart Secondary® sdAb-based secondary reagent conjugated to Janelia Fluor® 635b for detection of conventional primary antibodies, with spontaneous blinking in PBS, suitable for super-resolution microscopy including SMLM.

A Smart Secondary® sdAb-based secondary reagent conjugated to Janelia Fluor® 635b for detection of conventional primary antibodies, with spontaneous blinking in PBS, suitable for super-resolution microscopy including SMLM.

A Smart Secondary® sdAb-based secondary reagent conjugated to Janelia Fluor® 635b for detection of conventional primary antibodies, with spontaneous blinking in PBS, suitable for super-resolution microscopy including SMLM.

A fluorophore-conjugated single-domain antibody that specifically recognizes CD45 protein for direct and highly specific fluorescent detection.

A species-specific FluoTag® directed against IgG2a and IgG2b subtype from Mouse, serving as a fluorescent secondary reagent.

A fluorophore-conjugated single-domain antibody that specifically recognizes mouse and rat PSD95 for direct and highly specific detection.

A fluorophore-conjugated single-domain antibody specifically recognizing the luminal/extracellular domain of Synaptotagmin 1 (Syt1).

A fluorophore-conjugated single-domain antibody that specifically recognizes the cytoplasmic domain of murine and rat Synaptotagmin 1 (Syt1).

FluoTag®-Q anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. It features one fluorophore per sdAb molecule for more quantitative measurements (Q).

FluoTag®-Q anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. It features one fluorophore per sdAb molecule for more quantitative measurements (Q).

A fluorophore-conjugated single-domain antibody that strongly binds to the nucleocapsid (N) protein of SARS-CoV-2. Derived from a single-domain antibody (sdAb) that binds to the Nucleocapsid (N) protein of SARS-CoV-2 with high affinity, suitable for immunostaining and diagnostic marker applications.

FluoTag®-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed, and other DsRed derivatives with high affinity and specificity. Conjugated to fluorophores, it enables direct and highly specific fluorescent detection, eliminating the need for secondary antibodies.

A Smart Secondary® sdAb-based secondary reagent conjugated to Janelia Fluor® 635b for detection of conventional primary antibodies, with spontaneous blinking in PBS, suitable for super-resolution microscopy including SMLM.

Single-domain antibodies developed by NanoTag Biotechnologies targeting CD56 (Neural Cell Adhesion Molecule), a type I transmembrane glycoprotein expressed on neurons, glia, skeletal muscle, and natural killer cells, with applications in immunotherapy and cancer targeting.

Single-domain antibodies developed by NanoTag Biotechnologies targeting human CD19, a 95 KDa glycoprotein expressed in B lineage cells, used as a biomarker for B lymphocyte development and lymphoma diagnosis.

Single-domain antibodies developed by NanoTag Biotechnologies targeting human CD45, a type I transmembrane protein involved in T- and B-cell antigen receptor signaling, used in flow cytometry and immunohistochemistry.

Single-domain antibodies developed by NanoTag Biotechnologies targeting CD127 (Interleukin-7 Receptor subunit alpha), involved in T and B cell proliferation and associated with diseases like SCID and leukemia.

Single-domain antibodies developed by NanoTag Biotechnologies targeting Human Serum Albumin (HSA), the most abundant protein in human blood plasma, important for drug transport and osmotic regulation.

Single-domain antibodies developed by NanoTag Biotechnologies targeting CD62L (L-Selectin), a glycosylated transmembrane protein involved in lymphocyte homing.

Single-domain antibodies developed by NanoTag Biotechnologies targeting Tumor necrosis factor-alpha (TNFa), a cytokine involved in inflammatory responses and autoimmune disorders.

Single-domain antibodies developed by NanoTag Biotechnologies targeting CTLA-4, a protein on T lymphocytes that regulates immune response.

A recombinant heavy-chain anti-ALFA antibody genetically fused to guinea pig IgG Fc domain, preserving specificity and high-affinity binding of the anti-ALFA single-domain antibody. Suitable for Western blotting and immunodetection applications such as IF, IHC, and IP.

The recombinant heavy-chain anti-Syt1 Cytosolic Domain antibody (7D5) is a genetic fusion of camelid anti-Synaptotagmin 1 single-domain antibody to a heavy-chain antibody format.

A recombinant heavy-chain antibody (rHcAb) format of a single-domain antibody fused to an immunoglobulin Fc domain, providing a divalent binder with the same specificity as the nanobody.

A recombinant heavy-chain antibody (rHcAb) format of a single-domain antibody fused to an immunoglobulin Fc domain, providing a divalent binder with the same specificity as the nanobody.

Well-established single-domain antibodies targeting SYT1 and CNPase, available as Mouse or Rabbit IgG Fc fusions, combining nanobody-level specificity with enhanced detection flexibility and signal amplification.

The recombinant heavy-chain anti-Synaptotagmin 1 antibody (A51) is a genetic fusion of our camelid anti-Synaptotagmin 1 single-domain antibody (sdAb).

The recombinant heavy-chain anti-Synaptotagmin 1 antibody (A51) is a genetic fusion of our camelid anti-Synaptotagmin 1 single-domain antibody (sdAb).

An alpaca single-domain antibody (sdAb) that specifically recognizes the cytoplasmic domain of Synaptotagmin 1.

A recombinant heavy-chain anti-ALFA antibody genetically fused to mouse IgG2 Fc domain, preserving specificity and high-affinity binding of the anti-ALFA single-domain antibody. Suitable for Western blotting and immunodetection applications such as IF, IHC, and IP.

A recombinant heavy-chain anti-ALFA antibody genetically fused to mouse IgG1 Fc domain, preserving specificity and high-affinity binding of the anti-ALFA single-domain antibody. Suitable for Western blotting and immunodetection applications such as IF, IHC, and IP.

Single-domain antibody conjugated with Alexa Fluor 568 that specifically binds to mNeonGreen, used for immunostaining in fixed cells.

Specialized affinity resin designed for efficient purification and enrichment of Halo-tagged proteins using a high-affinity single-domain antibody (sdAb) that specifically recognizes the HaloTag®. Available as agarose or magnetic agarose beads.

Set of single-domain antibodies recognizing conventional immunoglobulins with species and isotype specificity, enhancing antibody-based assays in research and diagnostics.

Camelid single-domain antibodies conjugated to chemical fluorophores with defined fluorophore numbers and positions, available as FluoTag®-Q (one fluorophore) or FluoTag®-X2/X4 (multiple fluorophores) for quantitative and reliable fluorescence readouts.

GST Selector is based on a high-affinity single-domain antibody (sdAb) covalently immobilized on 4% cross-linked agarose beads, designed for selective binding of GST fusion proteins with high affinity and capacity.

Fusions of the sdAb anti-ALFA (NbALFA) and a secondary single-domain antibody (2°sdAb) recognizing primary antibodies, enabling simultaneous binding of ALFA-tagged effectors and primary IgG antibodies from rabbits or mice for modular ternary effector complexes.

MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. It provides high affinity and superior capacity for MBP fusion proteins with negligible non-specific background.

Affinity resin for purification of TagFP-tagged proteins.

The Ubiquitin pan Selector is an affinity resin that captures most ubiquitin forms (monomeric and polyubiquitin chains) using a single-domain antibody recognizing ubiquitin irrespective of its linkage or multimeric state.

Affinity resin for purification of RFP-tagged proteins.

Control resin for selector resin experiments.

Fusion proteins combining two single-domain antibodies targeting different or same sites to create adaptor molecules or increase binding affinity, with design and expression support in prokaryotic and eukaryotic systems.

Affinity resin for purification of mNeonGreen-tagged proteins.

Affinity resin for purification of GFP-tagged proteins.

Affinity resin for purification of ubiquitin K48-linked proteins.

Species and isotype-specific secondary nanobodies allowing multi-color fluorescent assays with multiple primary antibodies from the same species, enabling time-saving OneStep protocols and increased imaging resolution.

AA 1-419 from SARS-CoV-2 Nucleocapsid protein (UniProt ID: P0DTC9) fused to a C-terminal polyhistidine tag.

Affinity resin for purification of ubiquitin K63-linked proteins.

A nanobody library sourced from non-immunized animals offering a broad and diverse repertoire for targeting a wide range of antigens without prior immunization.

Technology equipping single-domain antibodies with fixation (FX) cassettes containing multiple primary amines to improve post-fixation properties, beneficial for STORM, DNA-PAINT microscopy, and expansion microscopy (ExM).

MiniSpin columns for use with selector resins, 50 units per pack.

Method combining species-specific secondary nanobodies with primary antibodies to prevent cluster formation, enabling pre-mixing or co-incubation for faster and higher resolution immunofluorescence and Western assays.

A synthetic nanobody library combining ribosome display and phage display technologies for rapid, animal-free identification of high-affinity nanobody candidates.

A complete nanobody discovery project including immunization, library construction, panning, and clone delivery within four to five months, tailored to specific project needs.

Proprietary technology for rapid and efficient affinity selection of target-specific B lymphocytes from PBMCs, speeding up single-domain antibody candidate selection with controlled avidity/affinity.

Protein expression and purification services in bacterial or mammalian systems, including animal-free growth media and endotoxin removal.

SyliteD1.1 anti-Gephyrin is a short dimeric peptidic probe that specifically binds gephyrin in inhibitory synapses.

A control protein consisting of a GFP variant tagged with a C-terminal ALFA-tag.

The anti-ALFA polyclonal antibody raised in rabbits recognizes the ALFA-tag (SRLEEELRRRLTE).

A control protein consisting of FLAG, HA, MBP, Myc, and ALFA tags.

Human B-cell Activator (hBCA) is a tetramerized single-domain antibody (sdAb) that crosslinks surface-bound human IgM to drive rapid activation.