Affina Biotechnologies
Affina Biotechnologies is dedicated to excellence in helping customers develop measurements of activities, kinetics, affinities of interactions, and concentrations of biomolecules. They offer a unique mix of label-free and label-dependent assay technologies supported by scientific and technical expertise and a wide range of instrumentation, including Surface Plasmon Resonance (SPR), light absorbance, luminescence, fluorescence polarization, and time-resolved fluorescence. The company specializes in developing custom-built measurements and screens, transferring validated methods to client facilities or performing them at Affina. Their services include affinity and kinetics of molecular interactions, enzyme activity, and total and active concentration. Affina aims to make contract research a rewarding experience for clients.
Industries
Nr. of Employees
small (1-50)
Affina Biotechnologies
Valhalla, New York, United States, North America
Patents
Services
Develop robust binding assays and inhibitor screens using label-free SPR or fluorescence-based formats (fluorescence polarization, TR-FRET), including affinity, kinetic and thermodynamic characterization and structure–activity relationship development.
Design and implement enzyme activity assays for protein characterization and inhibitor screening, including substrate selection, assay condition optimization, and determination of inhibition parameters (Ki, IC50, inhibition kinetics).
Measure total and active concentrations in biological samples using antibody-based assays (absorbance, fluorescence, TRF, FP) or SPR-based methods enabling sensitive or standard-free quantitation.
Provide protein expression in bacterial and baculovirus/insect systems, optimize expression conditions, purify soluble proteins or refold inclusion bodies, perform activity testing throughout purification, and remove endotoxin to client specifications.
Develop robust binding assays and inhibitor screens using label-free SPR or fluorescence-based formats (fluorescence polarization, TR-FRET), including affinity, kinetic and thermodynamic characterization and structure–activity relationship development.
Design and implement enzyme activity assays for protein characterization and inhibitor screening, including substrate selection, assay condition optimization, and determination of inhibition parameters (Ki, IC50, inhibition kinetics).
Measure total and active concentrations in biological samples using antibody-based assays (absorbance, fluorescence, TRF, FP) or SPR-based methods enabling sensitive or standard-free quantitation.
Provide protein expression in bacterial and baculovirus/insect systems, optimize expression conditions, purify soluble proteins or refold inclusion bodies, perform activity testing throughout purification, and remove endotoxin to client specifications.
Expertise Areas
- Assay development and validation
- Biomolecular interaction analysis and kinetics
- Enzymology and inhibitor screening
- Protein expression and purification
Key Technologies
- Surface plasmon resonance (SPR)
- Fluorescence polarization (FP)
- Time-resolved FRET (TR-FRET)
- Plate-based absorbance/fluorescence/luminescence detection
News & Updates
Announcement of the opening of new facilities in Westchester County, Elmsford, NY.
Announcement of the opening of new facilities in Westchester County, Elmsford, NY.