AAVnerGene
AAVnerGene is a leading biotech company specializing in advanced gene therapy technologies using the AAV platform. With over 20 years of expertise, they develop innovative solutions including AAV vector design, packaging, capsid library construction, and screening. Their proprietary ATHENA platform enables efficient identification and evolution of AAV capsids for targeted gene delivery, addressing challenges such as immune responses and high vector doses. The company collaborates with academic and biotech partners to push the frontiers of gene therapy, offering a comprehensive range of AAV products, services, and custom solutions to accelerate therapeutic development.
Industries
Nr. of Employees
small (1-50)
AAVnerGene
Rockville, Maryland, United States, North America
Products
Plasmid sets for single-, dual- and triple-plasmid production workflows
Transfer and helper plasmid sets formatted for single-, dual- and triple-plasmid AAV production workflows and for capsid/library construction.
Premade AAV viral vectors (multiple serotypes, promoters, reporters)
Ready-to-use AAV vectors supplied in multiple serotypes with defined genomes and characterized titers and QC for gene delivery in vitro and in vivo.
AAV reference materials (full, truly-empty, byproduct-empty)
Standardized AAV reference materials for assay calibration and QC, including fully packaged genomes, truly-empty capsids and byproduct-empty particles with documented characterization.
Capsid testing kits and barcode kits
Kits to enable capsid-barcode testing and pooled serotype/variant evaluation to support empirical selection using barcode sequencing and NGS.
Premade high-complexity capsid libraries
Ready-to-use capsid libraries with random peptide insertions at selected capsid variable regions, provided in multiple serotypes and library sizes for directed evolution.
Live-cell cAMP biosensor GPCR assay kits
Assay kits based on live-cell cAMP biosensors for rapid high-throughput functional screening of GPCRs.
Plasmid sets for single-, dual- and triple-plasmid production workflows
Transfer and helper plasmid sets formatted for single-, dual- and triple-plasmid AAV production workflows and for capsid/library construction.
Premade AAV viral vectors (multiple serotypes, promoters, reporters)
Ready-to-use AAV vectors supplied in multiple serotypes with defined genomes and characterized titers and QC for gene delivery in vitro and in vivo.
AAV reference materials (full, truly-empty, byproduct-empty)
Standardized AAV reference materials for assay calibration and QC, including fully packaged genomes, truly-empty capsids and byproduct-empty particles with documented characterization.
Capsid testing kits and barcode kits
Kits to enable capsid-barcode testing and pooled serotype/variant evaluation to support empirical selection using barcode sequencing and NGS.
Premade high-complexity capsid libraries
Ready-to-use capsid libraries with random peptide insertions at selected capsid variable regions, provided in multiple serotypes and library sizes for directed evolution.
Live-cell cAMP biosensor GPCR assay kits
Assay kits based on live-cell cAMP biosensors for rapid high-throughput functional screening of GPCRs.
Services
AAV vector design and optimization
Consultation, sequence design and construction of AAV transfer plasmids with selection of regulatory elements and optimization for target tissues and applications.
AAV packaging and contract manufacturing
Custom packaging of AAV vectors across multiple serotypes using plasmid-based production workflows with defined QC testing and turnaround options.
AAV analytical characterization and QC
Laboratory-based analytical testing for genome quantification, capsid titer, full/empty ratio, purity and standard safety endpoints to support release and downstream studies.
Capsid evaluation and optimization
Comparative testing of capsids and regulatory elements across cell types and animal models (in vitro and in vivo) to guide capsid selection for specific tissues and applications.
Capsid library production and directed evolution
Design, construction, production and selection/evolution of barcoded and random-insertion capsid libraries with support for pooled NGS-based screening in small and large animal models.
AAV experiment consultation and project support
Project consulting covering experimental planning, construct design, transfection and assay configuration support for AAV-based studies.
AAV vector design and optimization
Consultation, sequence design and construction of AAV transfer plasmids with selection of regulatory elements and optimization for target tissues and applications.
AAV packaging and contract manufacturing
Custom packaging of AAV vectors across multiple serotypes using plasmid-based production workflows with defined QC testing and turnaround options.
AAV analytical characterization and QC
Laboratory-based analytical testing for genome quantification, capsid titer, full/empty ratio, purity and standard safety endpoints to support release and downstream studies.
Capsid evaluation and optimization
Comparative testing of capsids and regulatory elements across cell types and animal models (in vitro and in vivo) to guide capsid selection for specific tissues and applications.
Capsid library production and directed evolution
Design, construction, production and selection/evolution of barcoded and random-insertion capsid libraries with support for pooled NGS-based screening in small and large animal models.
AAV experiment consultation and project support
Project consulting covering experimental planning, construct design, transfection and assay configuration support for AAV-based studies.
Expertise Areas
- AAV transfer vector design and construct optimization
- Plasmid-based AAV manufacturing and scale-up (single/dual/triple plasmid workflows)
- Capsid engineering including directed evolution, peptide insertion libraries and DNA shuffling
- High-complexity capsid library generation and barcoded pooled screening
Key Technologies
- Next-generation sequencing (barcode-seq) for pooled screens
- Barcoded capsid libraries
- Random-peptide insertion libraries (NNK insertion) with >1e9 complexity
- DNA shuffling / recombination libraries