Antimicrobial preparation and uses thereof
Inventors
Ala'Aldeen, Dlawer • Mahdavi, Jafar • Soultanas, Panos
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-9975914-B2
Publication Date
2018-05-22
Expiration Date
Abstract
A method of enhancing the growth of an animal is provided. The method includes causing the animal to ingest or absorb an effective amount of a preparation obtainable by a process including, bringing a tyrosinate moiety into contact with an Fe(III)-containing substance, optionally wherein the tyrosinate moiety is tyrosine and it is brought into contact with the Fe(III)-containing substance in the presence of a base, or wherein the tyrosinate moiety is a tyrosine salt and it is brought into contact with an Fe(III)-containing substance. Methods for inhibiting, reducing, or preventing biofilm formation or buildup on a surface; the treatment of, inhibition of growth of, and inhibition of colonization by, bacteria, both in biological and non-biological environments; disinfecting surfaces, potentiating the effects of antibiotics and other anti-microbial agents, and increasing the sensitivity of bacteria and other microorganisms, to anti-microbial agents are also provided.
Core Innovation
The invention relates to Fe(III)-tyrosine-derived antimicrobial preparations and complexes, including an Fe(III)-tyrosine complex formed by bringing a tyrosinate moiety into contact with an Fe(III)-containing substance in the presence of a base. The preparation is described as Fe–Tyr coordination between an Fe(III) source and a tyrosinate moiety, and may include μ-oxo trimeric complex forms. The described compositions provide antimicrobial activity connected to preventing biofilm formation and reducing bacterial growth and colonization.
A stated problem is that bacterial biofilm formation and bacterial colonization resist antimicrobial treatment, including in antibiotic-resistant contexts, and that reducing biofilm formation and colonization on biological and non-biological surfaces is needed. The disclosed Fe(III)-tyrosine complexes are described as inhibiting biofilm formation and as having roles in biofilm dissolution/dispersal. The disclosure further links these effects to potentiating antibiotics and reversing antibiotic resistance.
In the context of the independent claim, an animal feed comprising an Fe(III)-tyrosine complex is provided in an effective amount to reduce the number of Campylobacter present in the gastrointestinal tract of an animal. The Fe(III)-tyrosine complex is prepared by contacting a tyrosinate moiety with an Fe(III)-containing substance in the presence of a base, where the base is an amine or alkali metal hydroxide. The dependent claims refine the composition and preparation, including specific tyrosinate and Fe(III) source limitations and exclusions of isolating Fe(tyrosine)3.
Claims Coverage
The document contains one independent claim focused on an animal feed formulation and its preparation, with multiple dependent claims refining the complex composition, preparation characteristics, and antimicrobial-agent constraints.
Animal feed with Fe(III)-tyrosine complex to reduce Campylobacter
An animal feed comprising an Fe(III)-tyrosine complex in an effective amount to reduce the number of Campylobacter present in the gastrointestinal tract of an animal, where the Fe(III)-tyrosine complex is prepared by a method comprising bringing a tyrosinate moiety into contact with an Fe(III)-containing substance in the presence of a base, and the base is an amine or alkali metal hydroxide.
Specified L-tyrosine/L-tyrosinate fraction
The animal feed includes a tyrosine or tyrosinate component where the tyrosine or L-tyrosinate moiety makes up at least 30%.
Fe(III) source as Fe(OH)3
The Fe(III)-containing substance is Fe(OH)3.
Omission of isolating Fe(tyrosine)3
The method omits any step of isolating Fe(tyrosine)3 from components of the reaction mixture.
Non-therapeutic antimicrobial agent constraint
The animal feed includes one or more antimicrobial agents in an amount or concentration that is at least 5%, but below a therapeutically effective amount or concentration compared to when those agents are administered to a patient or animal not receiving the feed product.
Bringing step about 5 minutes
The bringing step occurs in about 5 minutes.
Overall claim coverage centers on using an Fe(III)-tyrosine complex in an animal feed to reduce gastrointestinal Campylobacter, with refinements specifying the tyrosinate fraction, Fe(III) source, exclusion of isolating Fe(tyrosine)3, and additional formulation constraints regarding antimicrobial-agent amounts and reaction/contact timing.
Stated Advantages
Reduces the number of Campylobacter present in the gastrointestinal tract of an animal.
Potentiates antibiotics.
Reverses antibiotic resistance.
Inhibits biofilm formation on surfaces.
Supports biofilm dissolution/dispersal.
Reduces bacterial growth and bacterial colonization.
Documented Applications
Animal feed use to reduce Campylobacter present in an animal gastrointestinal tract.
Inhibition of biofilm formation on biological and non-biological surfaces, including biofilm inhibition on bead/plastic/teeth [procedural detail omitted for safety].
Disinfecting/coating surfaces and medical devices [procedural detail omitted for safety].
Treating or inhibiting bacterial growth and colonization, including effects against antibiotic-resistant contexts [procedural detail omitted for safety].
Potentiating antibiotics and reversing antibiotic resistance [procedural detail omitted for safety].
A chicken study showing improved growth and reduced Campylobacter carriage using FeQ/FeTyr [procedural detail omitted for safety].
Interested in licensing this patent?