Sample test method, microfluidic device, and test device
Inventors
Park, Sung Ha • PARK, Sang Bum • Lee, Beom Seok • Kim, Kui Hyun • PARK, Joo Hee • SONG, Kyung Mi • CHO, Euy Hyun • Kim, Ha Na
Assignees
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Publication Number
US-9927351-B2
Publication Date
2018-03-27
Expiration Date
Abstract
A sample test method, microfluidic device, and test device efficiently and accurately compensates for interference of an interfering substance present in a sample using optical measurement without addition of a separate reagent for detecting the interfering substance. The sample test method includes: measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
Core Innovation
The invention relates to an in vitro sample test method in which an optical characteristic value of a target substance present in a sample is measured and an optical characteristic value of an interfering substance present in the sample is measured. A concentration of the target substance is determined for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance. The target substance interference compensation is performed using optical measurements of the interfering substance, without adding a separate reagent for detecting the interfering substance.
In the measurement of optical characteristic values, the method includes measuring optical characteristic values of the target substance at a main wavelength and a sub-wavelength, and subtracting the optical characteristic value at the sub-wavelength from the optical characteristic value at the main wavelength. The method also includes measuring optical characteristic values of the interfering substance at a plurality of wavelengths and determining a final optical characteristic value as a difference between two or more measured optical characteristic values measured at the plurality of wavelengths.
The invention is implemented in a test device that includes a measurer configured to measure optical characteristic values of a target substance and an interfering substance, and a data processor configured to determine a concentration of the target substance for which interference of the interfering substance is compensated based on the optical characteristic value of the interfering substance. The measurer measures optical characteristic values at a main wavelength and a sub-wavelength, and the data processor subtracts the optical characteristic value measured at the sub-wavelength from the optical characteristic value measured at the main wavelength.
Claims Coverage
The independent claims provide five inventive features covering a sample test method and corresponding test devices for compensating interference of an interfering substance using optical characteristic value measurements of both the target substance and the interfering substance.
Optical interference compensation using measured interfering substance optical characteristic value
A sample test method comprising measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
Main wavelength and sub-wavelength subtraction
Measuring optical characteristic values of the target substance at a main wavelength and a sub-wavelength, and subtracting the optical characteristic value at the sub-wavelength from the optical characteristic value at the main wavelength.
Plurality-of-wavelength difference for interfering substance
Measuring optical characteristic values of the interfering substance at a plurality of wavelengths and determining a final optical characteristic value as a difference between two or more measured optical characteristic values, where the final optical characteristic value is an optical characteristic value for the interfering substance.
Test device with measurer and data processor
A test device comprising a measurer configured to measure an optical characteristic value of a target substance present in a sample and an optical characteristic value of an interfering substance present in the sample, and a data processor configured to determine a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
Test device main wavelength and sub-wavelength subtraction
The measurer is configured to measure optical characteristic values of the target substance at a main wavelength and a sub-wavelength, and the data processor is configured to subtract the optical characteristic value measured at the sub-wavelength from the optical characteristic value measured at the main wavelength.
Multi-wavelength difference for interfering substance in a test device
The measurer is configured to measure optical characteristic values of the sample at a plurality of wavelengths, and the data processor is configured to determine a final optical characteristic value as a difference between two or more measured optical characteristic values measured at the plurality of wavelengths, where the final optical characteristic value is an optical characteristic value for the interfering substance.
The claims center on measurement-based interference compensation: measuring optical characteristic values for both the target substance and the interfering substance, computing difference-based optical characteristic values using either main wavelength/sub-wavelength subtraction or a plurality-of-wavelength difference for the interfering substance, and determining the target substance concentration for which the interference is compensated based on the interfering substance optical characteristic value.
Stated Advantages
Improved correlation/accuracy after interference compensation, including improved correlation coefficient and reduced false positives.
Reduced interference effect for other analytes when compensating for bilirubin interference, including improved chloride results after bilirubin interference compensation.
Documented Applications
In vitro diagnostic sample testing using optical measurements with interference compensation based on an interfering substance.
Blood samples with gamma-glutamyl transferase (GGT) as a target substance, including compensation for bilirubin interference.
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