Methods of reducing salmonella in poultry
Inventors
Cook, Mark Eric • Sand, Jordan Marshall • KOGUT, MIKE H. • SWAGGERTY, CHRISTINA L.
Assignees
US Department of Agriculture USDA • Wisconsin Alumni Research Foundation
Publication Number
US-9821029-B2
Publication Date
2017-11-21
Expiration Date
2033-08-02
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Abstract
Described herein are methods of reducing Salmonella in the intestines of poultry in need thereof by administering to a poultry bird an effective amount of an interleukin-10 peptide or an isolated antibody that specifically binds an interleukin-10 peptide. Administering may be performed within 1 to 4 weeks of harvest of the poultry in order to reduce Salmonella transmission to human consumers. Also included herein are finishing feeds that include an interleukin-10 peptide or an isolated antibody that specifically binds an interleukin-10 peptide.
Core Innovation
The invention provides methods of reducing Salmonella in the intestines of poultry by administering an effective amount of an interleukin-10 peptide or an isolated antibody that specifically binds an interleukin-10 peptide within 1 to 4 weeks prior to harvest of the poultry. This administration can be performed by feeding the poultry a finishing feed composition comprising the interleukin-10 peptide or the isolated antibody with no added antibiotics.
The problem addressed by the invention stems from the increasing concern regarding the use of antibiotics in poultry feed. Antibiotics have been traditionally used to promote growth and reduce disease occurrence, including reducing Salmonella transmission to humans. However, the use of antibiotics leads to issues such as antibiotic-resistant bacterial strains and residual antibiotics in human food. Notably, controlling Salmonella, particularly the pathogen Salmonella enterica subspecies enterica serovar Enteritidis, is challenging because poultry can be asymptomatic carriers and current methods to test and reduce Salmonella contamination in poultry products have limitations.
The invention solves this problem by utilizing interleukin-10 peptides and anti-interleukin-10 antibodies as a novel strategy that reduces Salmonella burden in poultry intestines at the finishing phase prior to slaughter. This method counters Salmonella tolerance mediated by IL-10 signaling, thereby improving immune response without relying on antibiotics and reducing the potential transmission of Salmonella to human consumers through poultry meat.
Claims Coverage
The patent includes one independent claim focused on treating poultry to reduce intestinal Salmonella using isolated antibodies against specific interleukin-10 peptides, including their forms and administration methods.
Method of reducing Salmonella using isolated antibodies binding specific interleukin-10 peptides
Administer to poultry an effective amount of an isolated antibody that specifically binds an interleukin-10 peptide selected from SEQ ID NO: 1 to SEQ ID NO: 5 within 1 to 4 weeks of harvest to reduce intestinal Salmonella.
Administration timing and scope
The method applies to poultry that may be Salmonella carriers and includes chickens, turkeys, ducks, quail, and pheasants, with administration timed according to bird type and age.
Forms and routes of administration
The isolated antibody can be administered orally or by injection, including as avian egg yolk antibodies and incorporated into poultry feed compositions, particularly antibiotic-free finishing feeds.
Antibody concentration in feed
The isolated antibody may be present in poultry feed compositions in an amount ranging from 0.15 mg/kg to 100 mg/kg of feed.
The claims focus on a novel antibiotic-free approach using isolated antibodies targeting specific IL-10 peptides to reduce poultry intestinal Salmonella burden effectively by administration within weeks prior to harvest, including incorporation in finishing feeds.
Stated Advantages
Reduces Salmonella burden in poultry intestines effectively during the finishing period before slaughter.
Provides an antibiotic-free method to reduce Salmonella transmission from poultry to humans.
Eliminates incubatory or convalescent carrier status for Salmonella in poultry.
Enables use in finishing feeds without added antibiotics, preventing antibiotic residues in poultry meat.
Egg yolk antibodies offer an inexpensive and safe source of antibodies for oral administration.
Documented Applications
Reducing Salmonella in intestines of broiler chickens administered at weeks 3 to 8 after hatch to prevent transmission to human consumers.
Reducing Salmonella in turkeys administered at weeks 8 to 22 after hatch as a feed additive or antibody in finishing diets prior to slaughter.
Use of anti-IL-10 antibody-containing finishing feeds without added antibiotics during the final 1 to 4 weeks before poultry harvest.
Potential reduction of Salmonella contamination on turkey carcasses and ground turkey meat to meet USDA testing standards.
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