Bioactive heme-haloperoxidase compositions and methods of their use
Inventors
Stephens, JR., Jackson T. • Pete, Matthew J.
Assignees
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Publication Number
US-9814234-B2
Publication Date
2017-11-14
Expiration Date
Abstract
Compositions comprising a heme-haloperoxidase and an amino alcohol effective for killing or inhibiting the growth of pathogenic microorganisms in the presence of a peroxide and halide and methods of use are provided.
Core Innovation
The invention relates to antimicrobial compositions for killing or inhibiting the growth of pathogenic microorganisms that include a mammalian eosinophil peroxidase, a peroxide or a peroxide source, and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine. The compositions are effective for killing or inhibiting pathogenic microorganisms, including microorganisms implicated in human or animal infection sites.
In the described compositions, the peroxide component is provided as hydrogen peroxide or via a peroxide source, including peroxide-producing oxidases. Halides can be included with the peroxide and amino alcohol components, and hypohalite and singlet molecular oxygen (1O2) are generated as part of the antimicrobial action. The amino alcohol enhances microbicidal activity of the eosinophil peroxidase.
The invention further includes binary formulations in which a first composition contains the mammalian eosinophil peroxidase and the amino alcohol, while a second composition contains peroxide or a peroxide-producing oxidase substrate. These binary formulations are effective when the first and second compositions are combined in a single solution and come into contact with pathogenic microorganisms, or when the first and second solutions are applied concurrently or sequentially to pathogenic microorganisms.
Claims Coverage
The partial claim set includes multiple independent claims that collectively cover single-composition antimicrobial formulations, compositions with halide and amino alcohol enhancement, method claims for contacting microorganisms and treating microbial infection in a human or animal subject, and binary formulations delivered together, concurrently, or sequentially. Across these independent claims, the core inventive features are the same component set: a mammalian eosinophil peroxidase combined with a peroxide or peroxide source and a specified amino alcohol, with halide and peroxide-producing oxidase/substrate arrangements in additional claim structures.
Eosinophil peroxidase with amino alcohol, peroxide or peroxide source
A composition for killing or inhibiting the growth of pathogenic microorganisms comprising a mammalian eosinophil peroxidase, a peroxide or a peroxide source, and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine.
Eosinophil peroxidase with halide and amino alcohol that enhances microbicidal activity
A composition for killing or inhibiting the growth of pathogenic microorganisms comprising a mammalian eosinophil peroxidase; a peroxide or a peroxide source; a halide; and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine, wherein the amino alcohol enhances the microbicidal activity of the eosinophil peroxidase.
Contacting microorganisms with eosinophil peroxidase plus amino alcohol in presence of peroxide and halide
A method for killing or inhibiting the growth of pathogenic microorganisms comprising contacting the microorganisms, in the presence of a peroxide and a halide, with an effective amount of a composition comprising a mammalian eosinophil peroxidase and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine.
Treating infection by administering eosinophil peroxidase plus amino alcohol with peroxide and halide
A method of treating a microbial infection in a human or animal subject comprising administering to the site of infection, in the presence of a peroxide and a halide, an effective amount of a composition comprising a mammalian eosinophil peroxidase and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine.
Binary formulation with eosinophil peroxidase and amino alcohol plus separate peroxide
A binary formulation for killing or inhibiting the growth of pathogenic microorganisms comprising (a) a first composition comprising a mammalian eosinophil peroxidase and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine; and (b) a second composition comprising a peroxide, wherein the binary formulation is effective when the first composition and the second composition are combined in a single solution and come in contact with the pathogenic microorganisms.
Binary formulation with eosinophil peroxidase plus peroxide-producing oxidase and oxidase substrate
A binary formulation comprising (a) a first composition comprising a mammalian eosinophil peroxidase, a peroxide-producing oxidase, and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine; and (b) a second composition comprising a substrate for the peroxide-producing oxidase, wherein the binary formulation is effective when the first composition and the second composition are combined in a single solution and come in contact with the pathogenic microorganisms.
Binary formulation delivered concurrently as two solutions
A binary formulation comprising (a) a first composition comprising a mammalian eosinophil peroxidase, a peroxide-producing oxidase, and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine; and (b) a second composition comprising a substrate for the peroxide-producing oxidase, wherein the binary formulation is effective when the first composition and the second composition are each in solution form and are applied concurrently to the pathogenic microorganisms.
Binary formulation delivered sequentially as two solutions
A binary formulation comprising (a) a first composition comprising a mammalian eosinophil peroxidase, a peroxide-producing oxidase, and an amino alcohol selected from ethanolamine, diethanolamine, and propanolamine; and (b) a second composition comprising a substrate for the peroxide-producing oxidase, wherein the binary formulation is effective when the first composition and the second composition are each in solution form and are applied sequentially to the pathogenic microorganisms.
Across the independent claims in the partial content, the claim coverage centers on antimicrobial activity driven by combining a mammalian eosinophil peroxidase with a peroxide or peroxide source and a selected amino alcohol (ethanolamine, diethanolamine, or propanolamine). Additional independent claim structures extend coverage to compositions that include a halide with amino alcohol-enhanced microbicidal activity, and to binary formulations where peroxide or a peroxide-producing oxidase/substrate system is separated into first and second compositions delivered together, concurrently, or sequentially.
Stated Advantages
Selective killing or inhibition of pathogenic microorganisms.
Minimizing collateral effects to normal flora.
Enhanced microbicidal activity when the amino alcohol enhances the microbicidal activity of the eosinophil peroxidase.
Retention of microbicidal activity for extended times.
Documented Applications
Treating a microbial infection in a human or animal subject by administering the composition to the site of infection.
Killing or inhibiting growth of pathogenic microorganisms by contacting the microorganisms with an effective amount of the composition in the presence of a peroxide and a halide.
Use of binary formulations effective when the first and second compositions are combined into a single solution and come into contact with pathogenic microorganisms.
Use of binary formulations applied concurrently to pathogenic microorganisms as two solution forms.
Use of binary formulations applied sequentially to pathogenic microorganisms as two solution forms.
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