Device including biosensor and holder
Inventors
Assignees
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Publication Number
US-9791401-B2
Publication Date
2017-10-17
Expiration Date
Abstract
A device utilizing biosensors to enable rapid electrochemical sensing of one or more analytes in a container. The device comprises a holder which incorporates at least one reference electrode and at least one sensing electrode. The sensing electrode comprising an electrically conductive substrate which is coated in a first layer of a suitable electron acceptor and subsequently with a second layer incorporating a biorecognition molecule adsorbed or within a suitable electropolymer matrix or carrier.
Core Innovation
The invention provides a device for a container or support adapted to receive a biological sample for rapid electrochemical sensing of one or more analytes using biosensors. The device forms at least part of a closure for the container or support and includes a holder that holds at least one sensing electrode and at least one reference electrode, with the or each electrode held and protruding from a sample receiving surface of the holder.
The sensing electrode comprises an electrically conductive substrate comprising platinum, platinum alloy, gold, gold alloy, or carbon. A first layer on the substrate comprises an electron acceptor, and a second layer comprises a biorecognition molecule adsorbed or within an electropolymer matrix or carrier.
The described design further specifies an electron acceptor material such as Ruthenium Purple and optionally an intermediate layer between the electron acceptor and the electropolymer matrix or carrier. In some embodiments, the intermediate layer is polyaniline or a derivative with non-polar substituents, and the partial content also discusses packaging or kit features for detecting biomarker analytes in a single or disposable use context.
Claims Coverage
The independent claim coverage centers on a closure-integrated container device with protruding sensing and reference electrodes, and a sensing electrode architecture that combines an electron-acceptor first layer with a biorecognition molecule second layer in or with an electropolymer matrix or carrier. Only one independent claim is explicitly available in the provided content.
Closure-integrated container device with protruding electrodes
A device for a container or support adapted to receive a biological sample for rapid electrochemical sensing, where the device forms at least part of a closure and includes a holder holding at least one sensing electrode and at least one reference electrode, the or each electrode being held and protruding from a sample receiving surface of the holder.
Electron-acceptor first layer on conductive substrate
The or each sensing electrode comprises an electrically conductive substrate comprising platinum, platinum alloy, gold, gold alloy, or carbon, with a first layer on the substrate comprising an electron acceptor.
Biorecognition second layer in or with electropolymer matrix or carrier
The or each sensing electrode has a second layer comprising a biorecognition molecule adsorbed or within an electropolymer matrix or carrier.
Across the available independent claim content, the inventive aspects are the closure-integrated container/support device with sensing and reference electrodes protruding from the sample receiving surface, and the sensing electrode design using an electron-acceptor first layer on a conductive substrate with a biorecognition molecule second layer carried by or within an electropolymer matrix or carrier.
Stated Advantages
Rapid electrochemical sensing using biosensors.
Rapid multi-analyte measurement is discussed in the partial content.
Very small samples are discussed in the partial content.
Documented Applications
Monitoring of stroke is discussed in the partial content.
Foetal hypoxia monitoring is discussed in the partial content.
Schizophrenia diagnosis is discussed in the partial content.
Detection of biomarker analytes including purines such as hypoxanthine, adenosine, ATP, and inosine is discussed in the partial content.
Detection of biomarker analytes including lactate and glutamate is discussed in the partial content.
Detection of biomarker analytes including D-serine is discussed in the partial content.
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