Eosinophil peroxidase compositions and methods of their use
Inventors
Stephens, JR., Jackson T. • Pete, Matthew J.
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-9782459-B2
Publication Date
2017-10-10
Expiration Date
Abstract
Eosinophil peroxidase compositions and methods of their use for killing and/or inhibiting the growth of susceptible microorganisms are provided. The compositions include an eosinophil peroxidase, a peroxide or peroxide source, and amino acids glycine, L-alanine, and L-proline.
Core Innovation
The invention provides eosinophil peroxidase (EPO) antimicrobial compositions for killing or inhibiting the growth of non-sporular bacteria. The compositions include eosinophil peroxidase, glucose oxidase, and amino acids glycine, L-alanine, and L-proline, wherein the amino acids enhance the antibacterial activity of the eosinophil peroxidase.
The invention further specifies that a peroxide source is generated by the glucose oxidase system, using glucose as the substrate. This enables an EPO-containing antimicrobial system to operate in the presence of non-sporular bacteria. The provided content also states that halide cofactors are needed for microbiocidal activity, including chloride and bromide.
A binary combination concept is also described, where an EPO-containing first composition with glucose oxidase and the amino acids glycine, L-alanine, and L-proline is combined with a second composition comprising glucose. The combination is defined as effective for killing or inhibiting the growth of non-sporular bacteria when the first and second compositions are combined in a single solution and come in contact with the non-sporular bacteria, or when the compositions are applied concurrently or sequentially in solution form.
Claims Coverage
The independent claims are clm-00001, clm-00014, clm-00016, and clm-00017. Across these claims, the inventive features focus on an EPO/glucose oxidase/amino acid composition for non-sporular bacteria, and a binary two-part EPO component plus glucose component effective upon combination, concurrent application, or sequential application.
Eosinophil peroxidase with glucose oxidase and enhancing amino acids
A composition for killing or inhibiting the growth of non-sporular bacteria comprising eosinophil peroxidase, glucose oxidase, and amino acids glycine, L-alanine, and L-proline, wherein the amino acids enhance the antibacterial activity of the eosinophil peroxidase.
Binary combination by combining an EPO first composition with a glucose second composition in a single solution
A binary combination for killing or inhibiting the growth of non-sporular bacteria comprising a first composition comprising an eosinophil peroxidase, glucose oxidase, and amino acids glycine, L-alanine, and L-proline, wherein the amino acids enhance the antibacterial activity of the eosinophil peroxidase; and a second composition comprising glucose, wherein the binary combination is effective for killing or inhibiting the growth of non-sporular bacteria when the first composition and the second composition are combined in a single solution and come in contact with the non-sporular bacteria.
Binary combination by concurrent application of solution-form first and second compositions
A binary combination for killing or inhibiting the growth of non-sporular bacteria, comprising a first composition comprising an eosinophil peroxidase, glucose oxidase, and amino acids glycine, L-alanine, and L-proline, wherein the amino acids enhance the antibacterial activity of the eosinophil peroxidase; and a second composition comprising glucose, wherein the binary combination is effective for killing or inhibiting the growth of non-sporular bacteria when the first composition and the second composition are each in solution form and are applied concurrently to the non-sporular bacteria.
Binary combination by sequential application of solution-form first and second compositions
A binary combination for killing or inhibiting the growth of non-sporular bacteria, comprising a first composition comprising an eosinophil peroxidase, glucose oxidase, and amino acids glycine, L-alanine, and L-proline, wherein the amino acids enhance the antibacterial activity of the eosinophil peroxidase; and a second composition comprising glucose, wherein the binary combination is effective for killing or inhibiting the growth of non-sporular bacteria when the first composition and the second composition are each in solution form and are applied sequentially to the non-sporular bacteria.
Overall, the claim coverage centers on an EPO plus glucose oxidase antimicrobial composition with the specific amino acids glycine, L-alanine, and L-proline claimed to enhance antibacterial activity against non-sporular bacteria, and binary combinations that pair an EPO first composition with a glucose second composition, with effectiveness defined by contact in a single combined solution or by concurrent or sequential solution-form application.
Stated Advantages
Killing or inhibiting the growth of non-sporular bacteria.
A binary combination is effective when combined into a single solution, and when applied concurrently or sequentially in solution form.
Amino acids glycine, L-alanine, and L-proline enhance the antibacterial activity of eosinophil peroxidase.
The approach is stated to be useful for infections including polymicrobial and multidrug-resistant infections.
Documented Applications
Topical in vivo treatment of surgical sites, including cancer surgical sites and colorectal and brain cancer sites.
In vitro disinfection/sterilization of biomedical devices (bandages, surgical instruments, suturing devices, catheters, dental appliances, contact lenses).
Use against polymicrobial infections and multidrug-resistant infections.
Treatment of non-sporular bacterial infections, including multidrug resistant non-sporular bacterial infections.
Interested in licensing this patent?