Method of identifying a biologically-active composition from a biofilm
Inventors
Berkes, Eva A. • Monsul, Nicholas T. • BOEHM, Frederick T.
Assignees
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Publication Number
US-9717765-B2
Publication Date
2017-08-01
Expiration Date
Abstract
The subject invention provides materials and methods that effectively support innate immunity and/or disperse pathogenic biofilms using readily available, nontoxic, natural substances, while supporting restoration of normal microbiotic homeostasis. In one embodiment, the subject invention provides anti-biofilm compositions comprising one or more probiotic organisms, anti-microbial honey, and other ingredients such as prebiotic compounds, other hive products, green tea derivatives, other plant derivatives, and vitamin D3.
Core Innovation
Described is a method for identifying and selecting a biologically-active composition from microbial biofilm cellular mass. The method comprises growing a microbial biofilm within a reactor vessel, contacting the biofilm with a biological growth medium, harvesting the biofilm by removing it from the reactor vessel to obtain a fraction comprising biofilm cellular mass, and testing the fraction for biological activity.
Also described is a method for preparing a biologically-active composition. The method comprises growing a microbial biofilm within a reactor vessel with a biological growth medium, harvesting the biofilm by removing it from the reactor vessel to obtain a fraction comprising biologically-active biofilm cellular mass, and combining the fraction with an appropriate carrier to form the biologically-active composition.
The described claim set includes limitations that the biological activity is anti-microbial activity and/or anti-inflammatory activity, and that the biofilm can be formed from probiotic microbes.
Claims Coverage
The document contains two independent claims covering core steps for obtaining biologically-active compositions from microbial biofilm cellular mass. Across the independent claims, the inventive features center on growing a microbial biofilm in a reactor vessel with a biological growth medium, harvesting the biofilm to obtain a fraction comprising biofilm cellular mass, testing and selecting an active fraction, and combining the biologically-active fraction with an appropriate carrier to form the composition.
Identifying and selecting a biologically-active composition from biofilm cellular mass
Growing a microbial biofilm within a reactor vessel, contacting the biofilm with a biological growth medium within the reactor vessel, harvesting the biofilm by removing the biofilm from the reactor vessel thereby obtaining a fraction comprising biofilm cellular mass, testing the fraction for biological activity, and selecting, as the biologically-active composition, a fraction that is found to have biological activity.
Preparing a biologically-active composition from biofilm cellular mass
Growing a microbial biofilm within a reactor vessel, wherein the biofilm is contacted with a biological growth medium within the reactor vessel, harvesting the biofilm by removing the biofilm from the reactor vessel thereby obtaining a fraction comprising biologically-active biofilm cellular mass, and combining the fraction with an appropriate carrier to form the biologically-active composition.
Across the two independent claims, the core coverage is based on reactor-grown microbial biofilms that are harvested into fractions comprising biofilm cellular mass, followed either by testing and selecting biologically-active fractions or by combining a biologically-active biofilm cellular mass fraction with an appropriate carrier to form the biologically-active composition. The claim set further narrows biological activity to anti-microbial and/or anti-inflammatory activity in dependent claims.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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