Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Assignees
Member
University of Nevada, Las VegasThe University of Nevada, Las Vegas is a public research university offering comprehensive undergraduate, graduate, and professional programs. The institution is recognized for research initiatives, interdisciplinary curriculum, community partnerships, and diverse student support. UNLV provides resources spanning financial assistance, technology, career development, outreach, and research, advancing individual achievement and community engagement in a multicultural environment.
The University of Nevada, Las Vegas is a public research university offering comprehensive undergraduate, graduate, and professional programs. The institution is recognized for research initiatives, interdisciplinary curriculum, community partnerships, and diverse student support. UNLV provides resources spanning financial assistance, technology, career development, outreach, and research, advancing individual achievement and community engagement in a multicultural environment.
Publication Number
US-9668479-B2
Publication Date
2017-06-06
Expiration Date
Abstract
Materials and methods for stimulating or inhibiting germination of Paenibacillus larvae spores, including methods for inhibiting P. larvae germination to reduce foulbrood in honey bees.
Core Innovation
The invention relates to preventing germination of Paenibacillus larvae spores associated with American foulbrood (AFB). It discloses contacting Paenibacillus larvae spores with indole- and phenol-derived compounds in an amount effective to prevent germination of the spores. The disclosure includes that Paenibacillus larvae spores require L-tyrosine and uric acid co-germinants for germination.
The disclosure further states that indole and phenol compounds inhibit germination of Paenibacillus larvae spores, including reported germination-inhibition activity with IC50 values. It describes compounds of Formula (I) and compounds of Formula (II), where substituents R and R1/R2 and integer parameters are defined, and it includes pharmaceutically acceptable salts of these compounds.
The document provides indole analog screening and indicates that halo-substituted indoles show enhanced inhibitory activity. It also discloses in vivo honey bee larval survival improvements when larvae diets are supplemented with indole analogs and larvae are challenged with Paenibacillus larvae spores, with germination assays and in vivo survival effects described in figures.
Claims Coverage
The document provides two independent claims directed to preventing germination of Paenibacillus larvae spores by contacting the spores with defined indole-based compounds. Across the independent claims, the inventive subject matter focuses on spore germination inhibition by specific compound classes and effective amounts for preventing germination.
Preventing spore germination by contacting with a compound of Formula (I)
A method for preventing germination of a Paenibacillus larvae spore by contacting the spore with a compound of Formula (I) or a pharmaceutically acceptable salt thereof, where each R is independently selected from unsubstituted (C1-C6)alkyl, unsubstituted (C2-C6)alkenyl, unsubstituted (C2-C6)alkynyl, halo, (C1-C6)haloalkyl, CN, and NO2, and n is an integer from 0 to 2, with the spore contacted with the compound in an amount effective to prevent germination.
Preventing spore germination by contacting with a selected indole compound
A method for preventing germination of a Paenibacillus larvae spore by contacting the spore with a compound selected from 4-cyanoindole, 5-bromindole, 5-chloroindole, 5-fluoroindole, 5-hydroxyindole, 5-nitroindole, 6-bromoindole, 6-chloroindole, 6-cyanoindole, and 6-fluoroindole, wherein the spore is contacted with the compound in an amount effective to prevent germination.
The independent claims collectively cover methods that inhibit Paenibacillus larvae spore germination by contacting the spores with either compounds defined by Formula (I) or one of a specified list of substituted indoles, each used at an amount effective to prevent germination.
Stated Advantages
Inhibits Paenibacillus larvae spore germination.
Improves honey bee larval survival when larvae diets are supplemented with indole analogs and challenged with Paenibacillus larvae spores.
Documented Applications
American foulbrood (AFB) control by preventing germination of Paenibacillus larvae spores.
Honey bee larval use in which larvae diets supplemented with indole analogs and challenged with Paenibacillus larvae spores show improved larval survival.
Interested in licensing this patent?