Magnetic nanoparticle-samirna complex and method for preparing same

Inventors

Chae, Jeiwook • Chung, Hyunjin • Lee, Boreum • Park, Han Oh

Abstract

Provided are a SAMiRNA-magnetic nanoparticle complex capable of effectively delivering a double-stranded oligo RNA and magnetic nanoparticles into a cell and a composition capable of simultaneously performing diagnosis and therapy of diseases such as cancer, and the like, containing the same. More specifically, provided is the SAMiRNA-magnetic nanoparticle complex consisting of double-stranded oligo RNA-polymer structures in which a hydrophilic material and a second hydrophobic material are bound to the double-stranded oligo RNA by a simple covalent bond or a linker-mediated covalent bond, and the magnetic nanoparticles in which a first hydrophobic material is bound onto a surface of the magnetic material, as a core.The SAMiRNA-magnetic nanoparticle complex may have a homogeneous size by a hydrophobic interaction between the first hydrophobic material of the present invention and the second hydrophobic material of the double-stranded oligo RNA structure.In addition, the hydrophilic material and the second hydrophobic material bound to the double-stranded oligo RNA structure may improve in vivo stability of the double-stranded oligo RNA, an additionally bound ligand may deliver the SAMiRNA-magnetic nanoparticle complex into a target cell even at a relative low concentration of dosage, and the magnetic materials of the magnetic nanoparticles may be used as an imaging agent for diagnosis.

Core Innovation

The invention relates to a SAMiRNA(s)-magnetic nanoparticle(s) complex comprising magnetic nanoparticle(s) including a first hydrophobic material coated on a surface of a magnetic material. The complex further includes a structure comprising second hydrophobic material, double stranded oligo RNA, and hydrophilic material, represented by Formula (1) as A-X—R—Y—B.

In the structure, one of A and B is the hydrophilic material and the other is the second hydrophobic material, while X and Y are simple covalent bonds or linker-mediated covalent bonds, and R is the double-stranded oligo RNA. The magnetic nanoparticle is positioned in a core by hydrophobic interaction between the first hydrophobic material and the second hydrophobic material, and the structure is positioned in a shell.

The hydrophilic material of the structure is bound to the outermost side of the double-stranded oligo RNA, so that the double-stranded oligo RNA is located within the shell portion bound to the outermost hydrophilic material. Optional ligand-containing variants support target-specific interaction described as receptor-mediated endocytosis (RME), where the ligand is bound in a target-specific manner.

Claims Coverage

The document provides one independent claim defining the SAMiRNA(s)-magnetic nanoparticle(s) complex with a core-shell assembly and a specific structure representation, and multiple dependent claims that refine features by specifying classes or ranges for materials and RNA, defining linkage degradability, and adding target-specific ligand functionality for receptor-mediated endocytosis (RME).

Overall, the claim set centers on a SAMiRNA-magnetic nanoparticle core-shell complex assembled by hydrophobic interaction, together with a Formula (1)-defined hydrophilic/hydrophobic RNA structure using simple or linker-mediated covalent bonds, further constrained by selectable hydrophobic coatings, RNA length, nanoparticle diameter, and optional degradable or non-degradable covalent linkages. Target-specific receptor-mediated endocytosis is incorporated through ligand selection.

Stated Advantages

Documented Applications