Transformant for production of lactic acid of high optical purity and method for producing lactic acid using the same
Inventors
Kim, Jae-Young • Shin, Sang-Heum • Chong, Hyon-Yong • Yang, Kap-Seok • Seo, Jeong-Sun
Assignees
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Publication Number
US-9428775-B2
Publication Date
2016-08-30
Expiration Date
Abstract
Disclosed is the biological production of lactic acid using a microorganism. A transformant capable of producing lactic acid of high optical purity at high yield, a method for the preparation thereof, and a method for producing lactic acid in a convenient and economically beneficial manner using the same are provided. The Zymomonas mobilis transformant can produce lactic acid of high optical purity at high yield without a stringent limitation to production conditions and a regulation of the intracellular metabolism pathway. Because it requires no additional separation and purification steps, the use of the transformant allows the production of lactic acid in a short process, resulting in a significant reduction in production cost, and avoiding the environment problems caused by precipitate wastes, which brings about environmental issues.
Core Innovation
The invention relates to a Zymomonas mobilis transformant comprising a gene encoding a D-lactate dehydrogenase derived from Leuconostoc sp. The encoded D-lactate dehydrogenase comprises the amino acid sequence of SEQ ID NO: 2, and the transformant is supported by introduced genetic information in Zymomonas mobilis.
The disclosure addresses production of D-lactic acid with high optical purity and high yield while maintaining performance under different pH conditions. The engineered Zymomonas mobilis transformant expresses the Leuconostoc-derived D-lactate dehydrogenase, and D-lactate dehydrogenase activity is elevated in engineered strains compared with relevant controls.
The disclosed engineered Zymomonas mobilis transformants produce D-lactic acid with high optical purity and improved characteristics across varied pH conditions, including pH-adjusted and pH-non-adjusted conditions. The disclosure further characterizes the transformants with respect to insertion-site independence and reduced byproducts compared with controls such as wild-type ZM4 and S. cerevisiae OC2.
Claims Coverage
The independent claim defines a Zymomonas mobilis transformant with one main inventive feature: expression of a Leuconostoc sp-derived D-lactate dehydrogenase defined by a specific SEQ ID NO: 2 amino acid sequence. Dependent claims further add refinement features specifying an exact nucleotide sequence (SEQ ID NO: 1) and an institutional deposit accession (KCTC 11803BP).
Leuconostoc sp-derived D-lactate dehydrogenase with SEQ ID NO: 2
A Zymomonas mobilis transformant comprising a gene encoding a D-lactate dehydrogenase derived from Leuconostoc sp, wherein the D-lactate dehydrogenase comprises the amino acid sequence of SEQ ID NO: 2.
D-lactate dehydrogenase gene with SEQ ID NO: 1
The Zymomonas mobilis transformant wherein the gene encoding the D-lactate dehydrogenase comprises the nucleotide sequence of SEQ ID NO: 1.
Deposited Zymomonas mobilis transformant under KCTC 11803BP
A Zymomonas mobilis transformant deposited under accession number KCTC 11803BP.
Overall claim coverage centers on a Zymomonas mobilis transformant engineered to express a Leuconostoc sp-derived D-lactate dehydrogenase defined by SEQ ID NO: 2, with dependent claim refinements for the exact gene nucleotide sequence (SEQ ID NO: 1) and an institutional deposit identification (KCTC 11803BP).
Stated Advantages
Produces D-lactic acid of high optical purity.
Produces D-lactic acid of high yield.
Maintains high optical purity across varied pH conditions (including pH-adjusted and pH-non-adjusted conditions).
Shows reduced byproducts compared with controls.
Documented Applications
Production of D-lactic acid using a Zymomonas mobilis transformant engineered with a Leuconostoc sp-derived D-lactate dehydrogenase under different pH conditions.
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