Diagnostic apparatus and diagnostic method using the same
Inventors
Jung, Jaean • Choi, Jae-Kyoung • Kim, Won-jung • KOO, Tae-Hee • Park, Min-A
Assignees
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Publication Number
US-9340818-B2
Publication Date
2016-05-17
Expiration Date
Abstract
Provided is a diagnostic apparatus. The diagnostic apparatus includes a microfluidic chip including first and second measurement parts for respectively measuring an amount of hemoglobin and an active degree of an enzyme within a blood sample. The second measurement part of the microfluidic chip analyzes the active degree of the enzyme within the blood sample using voltammetry.
Core Innovation
The invention is a diagnostic apparatus comprising a microfluidic chip with first and second measurement parts for measuring the amount of hemoglobin and the active degree of an enzyme within a blood sample. The chip includes a sample inlet port, a detergent-containing sample dissolution part that converts the blood sample into lysate, a passage to the measurement parts, and electrical lines connected to those parts.
The apparatus enables enzyme activity to be assessed relative to hemoglobin, particularly for glucose-6-phosphate dehydrogenase (G6PD), and reduces hematocrit-related error. The second measurement part includes an electrode and an electron transport medium. The described apparatus may also amplify, digitize, analyze, and display measurement results.
Claims Coverage
The supplied relevant claims identify one independent claim, which covers four main inventive features of a diagnostic apparatus and its microfluidic chip.
Separate measurement parts for hemoglobin and enzyme activity
The microfluidic chip comprises first and second measurement parts for respectively measuring an amount of hemoglobin and an active degree of an enzyme within a blood sample.
Detergent-containing sample dissolution part
The chip includes a sample inlet port and a sample dissolution part comprising detergent for converting the blood sample into lysate.
Passage and electrical connections
A passage moves the lysate into the first and second measurement parts, and lines are electrically connected to those measurement parts, respectively.
Electrode and electron transport medium
The second measurement part of the microfluidic chip comprises an electrode and an electron transport medium.
The independent claim covers all elements of the claim for a microfluidic diagnostic apparatus that measures hemoglobin and enzyme activity in a blood sample, including its sample dissolution, lysate passage, electrical connections, and second-measurement-part components.
Stated Advantages
Enables enzyme activity to be assessed relative to hemoglobin.
Reduces hematocrit-related error.
Documented Applications
Diagnostic measurement of blood hemoglobin and enzyme activity, particularly glucose-6-phosphate dehydrogenase (G6PD).
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