Purple transgenic fluorescent ornamental fish
Inventors
Blake, Alan • Crockett, Richard • Nasevicius, Aidas
Assignees
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Publication Number
US-9282729-B2
Publication Date
2016-03-15
Expiration Date
Abstract
The present invention relates to transgenic purple ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Core Innovation
The invention concerns transgenic tetra having a chromosomally integrated expression cassette encoding a fluorescent protein. The tetra comprises the Purple tetra 1 transformation event, and the sperm comprising the Purple tetra 1 transformation event has been deposited as ECACC accession no. 14011702.
The disclosed subject matter includes fertile transgenic progeny and derived life stages associated with the Purple tetra 1 transformation event, including stably expressing lines. Embodiments describe fluorescent protein transgenes that are chromosomally integrated, yielding transgenic tetra suitable for tracking embryonic tracing cell lineage and cell migration.
The disclosure further includes work related to genetic mosaic experiments and use of transgenic tetra as models involving fish cancer models.
Claims Coverage
The independent claims cover two inventive areas: a specific transgenic tetra defined by the chromosomally integrated fluorescent-protein expression cassette tied to the Purple tetra 1 transformation event using ECACC accession no. 14011702 sperm, and a method of producing a transgenic tetra by obtaining that event-associated tetra and breeding it with a second tetra. Across the independent-claim scope, the key inventive features are the named Purple tetra 1 transformation event, the ECACC-deposited sperm source, and the chromosomally integrated expression cassette encoding a fluorescent protein.
Chromosomally integrated fluorescent protein expression cassette tied to Purple tetra 1 transformation event and ECACC sperm deposit
A transgenic tetra comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the Purple tetra 1 transformation event, sperm comprising the Purple tetra 1 transformation event having been deposited as ECACC accession no. 14011702.
Producing a transgenic tetra by obtaining Purple tetra 1 event tetra and breeding with a second tetra
A method of producing a transgenic tetra comprising obtaining a tetra that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the Purple tetra 1 transformation event, sperm comprising the Purple tetra 1 transformation event having been deposited as ECACC accession no. 14011702; and breeding the obtained tetra with a second tetra to provide a transgenic tetra comprising the Purple tetra 1 transformation event.
Overall, the claim coverage is centered on transgenic tetra defined by a chromosomally integrated fluorescent-protein expression cassette associated with the Purple tetra 1 transformation event and ECACC accession no. 14011702 deposited sperm, and on producing further Purple tetra 1 event transgenic tetra by obtaining such tetra and breeding with a second tetra.
Stated Advantages
Improved visualization for scientific research utility.
Usefulness for scientific research applications including embryonic tracing cell lineage and cell migration, genetic mosaic experiments, and fish cancer models.
Documented Applications
Embryonic tracing cell lineage and cell migration using transgenic tetra with chromosomally integrated fluorescent transgenes.
Genetic mosaic experiments using the transgenic tetra.
Fish cancer models using the transgenic tetra.
Providing transgenic fish to the ornamental fish market and distributing the fish through grower/commercial distributor/retailer roles.
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