Method of producing native components, such as growth factors or extracellular matrix proteins, through cell culturing of tissue samples for tissue repair
Inventors
Assignees
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Publication Number
US-9220803-B2
Publication Date
2015-12-29
Expiration Date
Abstract
A medical composition is disclosed, which is injectable and which comprises a mixture of native components, which are obtainable by culturing one or more cell samples from a human or animal during normal conditions, said native components being included in the group consisting of growth factors, extracellular matrix proteins, and other substances produced by said cell samples during normal conditions, and a pharmaceutically acceptable carrier, as well as a method for producing the native components, a method for producing the medical composition, a method for treating a subject in need of tissue repair by injection of the medical composition, and use of said mixture of native components for the production of said medical composition for tissue repair via injection. A medical composition is disclosed, which is injectable and which comprises a mixture of native components, which are obtainable by culturing one or more cell samples from a human or animal during normal conditions, said native components being included in the group consisting of growth factors, extracellular matrix proteins, and other substances produced by said cell samples during normal conditions, and a pharmaceutically acceptable carrier, as well as a method for producing the native components, a method for producing the medical composition, a method for treating a subject in need of tissue repair by injection of the medical composition, and use of said mixture of native components for the production of said medical composition for tissue repair via injection.
Core Innovation
The invention provides a method for producing a mixture of native components, comprising growth factors, extracellular matrix proteins and other substances produced by cell samples during normal conditions. The native components are obtained by culturing cells in a nutrient medium in a bioreactor under rotating condition, wherein a culturing medium is obtained containing native components produced during the cell culturing. The method further includes drawing off the culturing medium at least once and separating the native components from the culturing medium to obtain the mixture of native components.
The drawing off is carried out continuously, and the drawn-off culturing medium is replaced with new nutrient medium. The bioreactor includes two or more interconnected chambers separated by a Millipore filter, allowing culturing medium containing native components to diffuse into a second chamber or third chamber and so on, and allowing for separation of the cells from the culturing medium containing native components in the bioreactor. Each cell sample originates from a tissue biopsy taken from cartilage and/or bone.
After separation, the separated native components can optionally be sterilized, enriched, and/or combined with a carrier and optionally freeze-dried. The separated native components are defined as mixtures of native components produced under normal culture conditions and can be combined with an injectable pharmaceutically acceptable carrier for use in tissue repair by injection.
Claims Coverage
The partial content provides one independent claim (clm-00001). The claim coverage centers on producing and recovering a mixture of native components using rotating, continuously operated, interconnected-chamber bioreactor culture and subsequent separation, with optional sterilizing, enriching, carrier combination, and freeze-drying, and a cartilage and/or bone biopsy cell source.
Rotating continuous bioreactor culture of biopsy-derived cells
A method including adding one or several different cell samples of human or animal origin to a bioreactor containing a nutrient medium, culturing during normal conditions under rotating condition, and continuously drawing off culturing medium including native components produced during the cell culturing while replacing the drawn-off culturing medium with new nutrient medium.
Interconnected chambers separated by Millipore filter for diffusion and cell separation
The bioreactor having two or more interconnected chambers separated by a Millipore filter which allows for the culturing medium containing native components produced to diffuse into a second chamber or third chamber and so on and for separation of the cells from the culturing medium containing native components in the bioreactor.
Separation of native components from culturing medium to obtain a mixture
Separating the native components from the culturing medium thereby obtaining a mixture of native components comprising growth factors, extracellular matrix proteins and other substances produced by the cell samples during normal conditions.
Optional sterilizing, enriching, carrier combination, and freeze-drying of separated native components
Optionally sterilizing, enriching, adding a carrier, and/or freeze-drying the separated native components.
Cartilage and/or bone tissue biopsy origin of each cell sample
Wherein each cell sample originates from a tissue biopsy taken from cartilage and/or bone.
Claim clm-00001 covers a method that cultures human or animal cells derived from cartilage and/or bone biopsies in a rotating bioreactor, continuously draws off culturing medium and replaces it with new nutrient medium, uses interconnected chambers separated by a Millipore filter to enable diffusion of medium with native components and separation of cells from that medium, separates native components to obtain a mixture, and optionally sterilizes, enriches, adds a carrier, and/or freeze-dries the separated native components.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Use for tissue repair by injection (injectable medical composition comprising native components and optionally a pharmaceutically acceptable carrier).
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