Method improve spatial memory via pterostilbene administration

Inventors

Joseph, James A.Rimando, Agnes M.Shukitt-Hale, Barbara

Assignees

US Department of Agriculture USDA

Publication Number

US-9028887-B2

Publication Date

2015-05-12

Expiration Date

2028-06-10

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Abstract

Disclosed is a pharmaceutical composition for treating oxidative stress comprising a therapeutically effective amount of a substantially pure compound of pterostilbene and a physiologically acceptable carrier. Pterostilbene is administered in an amount between about 2.5 mg to about 10 mg per kilogram of subject body weight. Also disclosed is a method for increasing a working memory of a subject, the method comprising administrating an effective amount of a substantially pure compound of pterostilbene, wherein the working memory for a subject increase and the therapeutic effectiveness is about 10 mg of pterostilbene per kilogram of subject body weight.

Core Innovation

The invention relates to a method of administering pterostilbene, a substantially pure compound and methylether analog of resveratrol, in therapeutically effective amounts to treat oxidative stress and improve neuronal and behavioral aging effects, particularly working memory and motor function. The composition includes pterostilbene with a physiologically acceptable carrier, administered in doses between about 2.5 mg to about 10 mg per kilogram of subject body weight. Pterostilbene is effective in reversing motor deficits and improving working memory in subjects, including senescent subjects.

The problem being solved is the need for compounds, particularly those present in berryfruits such as Vaccinium berries like blueberries, that can prevent, forestall, and reverse cognitive and motor deficiencies associated with aging and neurodegenerative diseases caused by oxidative stress. Existing antioxidants like resveratrol have anti-aging properties, but there was a need to identify other stilbene derivatives like pterostilbene that effectively protect against oxidative stress-related cognitive and motor decline. The invention addresses the need to identify compounds that enhance acetylcholine signaling, specifically muscarinic acetylcholine receptor activity, and protect dopamine release under oxidative stress.

Claims Coverage

The patent includes one independent claim focused on a method for increasing spatial learning by administering pterostilbene. There is a total of seven claims, with the first being the independent method claim, and the others depending on that claim and specifying details.

Method for increasing spatial learning using pterostilbene

Administering an effective amount of isolated or purified pterostilbene to a subject in need thereof such that the subject's spatial learning is increased.

Effective dosage of pterostilbene

An effective amount of pterostilbene is about 10 mg of the compound per kilogram of subject body weight for increasing spatial learning.

Pharmaceutical carrier administration

Pterostilbene is administered together with a pharmaceutical carrier.

Nutraceutical carrier administration

Pterostilbene is administered together with a nutraceutical carrier.

Range of pterostilbene dosage

The effective amount of pterostilbene ranges from about 2.5 mg to about 10 mg per kilogram of subject body weight.

Treatment of neurodegenerative diseases

The method is used where the subject is afflicted with a neurodegenerative disease.

Specific treatment for Alzheimer's disease

The neurodegenerative disease treated by pterostilbene administration includes Alzheimer's disease.

The independent claim covers the method of enhancing spatial learning by administering isolated or purified pterostilbene in effective dosages, including specific pharmaceutical or nutraceutical carriers, and for subjects afflicted by neurodegenerative diseases like Alzheimer's disease. The inventive features focus on the method of administration, dosage ranges, and application to cognitive disorders.

Stated Advantages

Pterostilbene supplementation reverses cognitive and motor deficiencies associated with aging in a dose-dependent manner.

Administration of pterostilbene protects muscarinic acetylcholine receptor activity against oxidative stress.

Pterostilbene antagonizes dopamine-induced decreases in calcium recovery in cells expressing M1 muscarinic receptors.

Dietary pterostilbene prevents dopamine release reduction under oxidative stress conditions.

Improved spatial memory and working memory performance in subjects administered pterostilbene.

Documented Applications

Treatment of oxidative stress to forestall, prevent, and reverse effects of neuronal and behavioral aging.

Improving working memory and motor function in aging and senescent subjects.

Treatment and prevention of neurodegenerative diseases including Alzheimer's disease.

Administration as a dietary supplement via Vaccinium berries, particularly blueberries.

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