Green transgenic fluorescent ornamental fish
Inventors
Blake, Alan • Crockett, Richard • Nasevicius, Aidas
Assignees
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Publication Number
US-8975467-B2
Publication Date
2015-03-10
Expiration Date
Abstract
The present invention relates to transgenic green ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Core Innovation
The invention provides transgenic tetra that comprise a chromosomally integrated expression cassette encoding a green fluorescent protein. The tetra comprises the “Green tetra 1 transformation event,” and sperm comprising the Green tetra 1 transformation event has been deposited as ECACC accession no. 12061301. The transgenic tetra thus link a specific transformation event to a deposited sperm resource and a chromosomal integration format.
A producing approach is provided for the transgenic tetra comprising the “Green tetra 1 transformation event.” The approach obtains a tetra that comprises a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the tetra comprises the “Green tetra 1 transformation event,” with sperm comprising the Green tetra 1 transformation event deposited as ECACC accession no. 12061301. The obtained tetra is then bred with a second tetra to provide a transgenic tetra comprising the Green tetra 1 transformation event.
In certain embodiments, the breeding uses a second tetra that is a non-transgenic tetra, thereby supporting maintenance or propagation of the Green tetra 1 transformation event through breeding with a non-transgenic partner. Additional embodiments define progeny fish as fertile and/or as a “transgenic White Tetra,” including progeny with genotype constraints for the integrated expression cassette, such as homozygous for the integrated cassette.
Claims Coverage
The provided independent claims cover two aspects: a transgenic tetra defined by a chromosomally integrated green fluorescent protein expression cassette linked to the “Green tetra 1 transformation event” and ECACC-deposited sperm, and a method of producing the same transgenic tetra by obtaining a tetra with that transformation event and breeding with a second tetra. Across these independent claims, the main inventive elements total two core feature sets, with the dependence refining partner type and progeny definitions.
Green tetra 1 linked chromosomal fluorescent expression cassette
A transgenic tetra comprising a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the tetra comprises the “Green tetra 1 transformation event,” and sperm comprising the Green tetra 1 transformation event has been deposited as ECACC accession no. 12061301.
Breeding-based production of Green tetra 1 transgenic tetra
A method of producing a transgenic tetra comprising obtaining a tetra that comprises a chromosomally integrated expression cassette encoding a green fluorescent protein, wherein the tetra comprises the “Green tetra 1 transformation event,” and sperm comprising the Green tetra 1 transformation event has been deposited as ECACC accession no. 12061301; and breeding the obtained tetra with a second tetra to provide a transgenic tetra comprising the Green tetra 1 transformation event.
Overall, the claim set ties the Green tetra 1 transformation event to a deposited ECACC sperm accession and a chromosomally integrated green fluorescent protein expression cassette, and it provides a breeding-based method to produce transgenic tetra containing the Green tetra 1 transformation event, with dependents further specifying non-transgenic breeding partners and progeny/genotype attributes.
Stated Advantages
Documented Applications
No documented applications found
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