Compositions for enhancing the antibacterial activity of myeloperoxidase and methods of use thereof
Inventors
Becquerelle, Sophie • Haag, William • Abril-Horpel, Obsidiana • Valvani, Shri
Assignees
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Publication Number
US-8945540-B2
Publication Date
2015-02-03
Expiration Date
Abstract
Methods and compositions are provided for inhibiting the growth of susceptible microorganisms by contacting the microorganisms, in the presence of a peroxide and chloride or bromide, with myeloperoxidase and at least two activity enhancing agent amino acids.
Core Innovation
The invention provides compositions and binary combinations that inhibit the growth of susceptible microorganisms using myeloperoxidase together with glucose oxidase. The compositions further include sodium chloride, polysorbate 80, phosphate buffer, and two amino acids selected so that one amino acid is glycine and the other enhances microbicidal activity in combination with the myeloperoxidase.
In the described compositions, the second amino acid is selected from D-isoleucine; L-isoleucine; L-tyrosine; and L-valine. The two amino acids work in combination to enhance the microbicidal activity of the myeloperoxidase.
The invention also includes binary combinations where a first composition contains myeloperoxidase, glucose oxidase, sodium chloride, polysorbate-80, phosphate buffer, water, and two amino acids with the glycine pairing and specified amino-acid selection, and where a second composition includes glucose and water. The two-part formulation is stated as a binary combination for killing or inhibiting the growth of susceptible microorganisms.
Claims Coverage
The independent claims cover three inventive features: a multi-component antimicrobial composition with myeloperoxidase, glucose oxidase, and specified formulation components; a composition using glycine, L-alanine, and L-proline with myeloperoxidase-enhancing microbicidal activity; and binary two-part combinations in which the first part contains the myeloperoxidase system and the second part contains glucose in water.
Myeloperoxidase-enhanced microbicidal composition with glycine and selected isoleucine/tyrosine/valine amino acid
A composition for inhibiting the growth of susceptible microorganisms comprising myeloperoxidase, glucose oxidase, sodium chloride, polysorbate 80, phosphate buffer, and two amino acids, wherein one of the two amino acids is glycine and the other is selected from D-isoleucine; L-isoleucine; L-tyrosine; and L-valine, and the two amino acids work in combination to enhance the microbicidal activity of the myeloperoxidase.
Myeloperoxidase-enhanced microbicidal combination of glycine, L-alanine, and L-proline
A composition for killing or inhibiting the growth of susceptible microorganisms consisting of myeloperoxidase, glycine, L-alanine, L-proline, glucose oxidase, sodium chloride, polysorbate-80, sodium phosphate buffer, and water, wherein the glycine, L-alanine, and L-proline work in combination to enhance the microbicidal activity of the myeloperoxidase.
Two-part binary combination with myeloperoxidase system plus glucose-in-water
A binary combination comprising a first composition comprising myeloperoxidase, glucose oxidase, sodium chloride, polysorbate-80, phosphate buffer, water and two amino acids, wherein one amino acid is glycine and the other is selected from D-isoleucine; L-isoleucine; L-tyrosine; and L-valine, and a second composition comprising glucose and water.
Two-part binary combination with glycine, L-alanine, and L-proline plus glucose-in-water
A binary combination for killing or inhibiting the growth of susceptible microorganisms consisting of a first composition consisting of myeloperoxidase, glycine, L-alanine, L-proline, glucose oxidase, sodium chloride, polysorbate-80, sodium phosphate buffer, and water, and a second composition comprising glucose and water.
Overall, the independent claims cover microbicidal compositions and binary combinations that include a myeloperoxidase system with glucose oxidase and specified buffering, salt, and surfactant components, together with glycine paired with selected amino acids or with the specific trio glycine, L-alanine, and L-proline.
Stated Advantages
Enhances the microbicidal activity of the myeloperoxidase.
Inhibits the growth of susceptible microorganisms.
Kills or inhibits the growth of susceptible microorganisms.
Documented Applications
Treating a microbial infection in a human or animal by administering, to the infection site, the composition described in the claims.
Treatment of infections characterized as polymicrobial.
Treating a microbial infection affecting gums, eyes, ears, skin, soft tissue, wounds, vaginal areas, groin areas, bed sores, or burn areas.
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