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Publication Number
US-8883717-B2
Publication Date
2014-11-11
Expiration Date
Abstract
Multilayer films comprised of polypeptide epitopes and a toll-like receptor ligand are described. The multilayer films are capable of eliciting an immune response in a host upon administration to the host. The multilayer films can include at least one designed peptide that includes one or more polypeptide epitopes from a virus, bacteria, fungus or parasite.
Core Innovation
The disclosed subject matter relates to immunogenic multilayer film vaccine compositions formed from a first multilayer film comprising a plurality of oppositely charged polyelectrolyte layers. One of the polyelectrolyte layers comprises a first antigenic polyelectrolyte that comprises a covalently linked viral, bacterial, fungal, or parasite epitope, and the multilayer film further comprises a toll-like receptor ligand (TLR ligand) covalently linked to the first antigenic polyelectrolyte.
In the described compositions, the TLR ligand is a lipoprotein or a lipopeptide and is covalently linked to the antigenic polyelectrolyte within the multilayer film. The polyelectrolytes comprise a polycationic material or a polyanionic material having a molecular weight of greater than 1,000 and at least 5 charges per molecule.
The disclosed embodiments include designed polypeptides and multilayer configurations that incorporate antigenic polypeptides and TLR ligands into the same multilayer structure. Additional embodiments include polypeptides and TLR ligands covalently attached to the same or different polyelectrolytes and incorporated into multilayer films, including multilayer deposition onto cores and multilayer mixing.
Additional embodiments include structural stabilization approaches for the multilayer films, including disulfide locking and covalent amide bond crosslinking.
Claims Coverage
The provided claim set includes one independent claim. The independent claim is directed to a multilayer film vaccine composition that combines a covalently linked antigenic polyelectrolyte with a covalently linked TLR ligand within a film made of oppositely charged polyelectrolyte layers, using polyelectrolyte materials with specified molecular weight and charge density.
Oppositely charged polyelectrolyte multilayer film with covalently linked antigenic polyelectrolyte epitopes
A first multilayer film comprising a plurality of oppositely charged polyelectrolyte layers, wherein one of the polyelectrolyte layers comprises a first antigenic polyelectrolyte that comprises a covalently linked viral, bacterial, fungal or parasite epitope.
Covalently linked TLR ligand as lipoprotein or lipopeptide to the antigenic polyelectrolyte
The multilayer film comprises a toll-like receptor ligand (TLR ligand) covalently linked to the first antigenic polyelectrolyte, wherein the TLR ligand is a lipoprotein or a lipopeptide.
Polyelectrolyte materials with molecular weight and charge requirements
The polyelectrolytes in the multilayer film comprise a polycationic material or a polyanionic material having a molecular weight of greater than 1,000 and at least 5 charges per molecule.
Overall, the independent claim requires an oppositely charged polyelectrolyte multilayer film that presents a covalently linked viral, bacterial, fungal or parasite epitope via an antigenic polyelectrolyte, with a covalently linked lipoprotein or lipopeptide TLR ligand associated to that antigenic polyelectrolyte, using polycationic or polyanionic polyelectrolytes with molecular weight and charge-per-molecule thresholds.
Stated Advantages
Documented Applications
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