Compositions and methods to promote implantation and engrafment of stem cells
Inventors
Assignees
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Publication Number
US-8871199-B2
Publication Date
2014-10-28
Expiration Date
Abstract
Tissue repair in-vivo depends on acute inflammation, but in many clinical situations the other major components of healing such as blood supply, anabolic hormones, growth factors, and stem cells are lacking. This invention includes compositions consisting of an agent which induces an inflammatory healing response combined with an autologous platelet lysate at a specific concentration which may have demonstrated in-vitro abilities to expand autologous tissue repair cells.
Core Innovation
The invention relates to a method for facilitated tissue repair in a subject in need thereof. The method includes obtaining platelets from the subject and preparing a 5% to 40% platelet lysate solution from the platelets.
The method further includes administering a hyper osmolar agent to the subject in an amount sufficient to induce local inflammation at the site in need of tissue repair. After administering the hyper osmolar agent, the method subsequently and non-contemporaneously administers a composition comprising the 5% to 40% platelet lysate solution to the subject at the site in need of tissue repair.
Tissue repair is facilitated at the site in the subject in need of tissue repair. The described approach further includes an optional timing relationship in which the non-contemporaneously administered composition containing the 5% to 40% platelet lysate solution is administered 3-5 days after the administration of the hyper osmolar agent.
Claims Coverage
The partial content provides one independent claim describing a facilitated tissue repair method, with dependent refinements. The independent claim contains four main inventive features: preparing a 5% to 40% platelet lysate solution, inducing local inflammation with a hyper osmolar agent, non-contemporaneously administering the platelet lysate composition, and facilitating tissue repair at the site.
Facilitated tissue repair using subject-derived platelet lysate
obtaining platelets from the subject and preparing a 5% to 40% platelet lysate solution from the platelets, then subsequently and non-contemporaneously administering a composition comprising the 5% to 40% platelet lysate solution to the subject at the site in need of tissue repair, wherein tissue repair is facilitated at the site in the subject in need of tissue repair
Hyper osmolar agent to induce local inflammation at the repair site
administering a hyper osmolar agent to the subject in an amount sufficient to induce local inflammation at the site in need of tissue repair
Non-contemporaneous administration of platelet lysate after inflammation induction
subsequently and non-contemporaneously administering a composition comprising the 5% to 40% platelet lysate solution to the subject at the site in need of tissue repair
Concentration-defined platelet lysate (5% to 40%)
preparing a 5% to 40% platelet lysate solution from the platelets; and subsequently administering a composition comprising the 5% to 40% platelet lysate solution at the site
Overall, the claim coverage centers on a facilitated tissue repair sequence: create local inflammation with a hyper osmolar agent and then non-contemporaneously deliver a subject-derived 5% to 40% platelet lysate solution to the tissue-repair site, with dependent refinements including a 3-5 day interval and other compositional or formulation elements described in the partial content.
Stated Advantages
Documented Applications
No documented applications found
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