Biofilm-removing antimicrobial compositions and uses thereof
Inventors
Gawande, Purushottam V. • Madhyastha, Srinivasa • LoVetri, Karen • Yakandawala, Nandadeva • Froehlich, Gord
Assignees
Publication Number
US-8753692-B2
Publication Date
2014-06-17
Expiration Date
2030-01-22
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Abstract
The present invention provides novel compositions comprising: (a) protamine sulfate and (b) benzalkonium chloride or a silver containing particle; or (a) sodium metaperiodate and (b) 5-fluorouracil or chlorhexidine and uses thereof for the preparation of devices, and in particular medical devices, susceptible to colonization by biofilm forming bacteria.
Core Innovation
The invention provides novel antimicrobial compositions for inhibiting or removing microbial biofilms, comprising combinations such as protamine sulfate and benzalkonium chloride or a silver containing particle; or sodium meta-periodate combined with 5-fluorouracil or chlorhexidine. These compositions are effective against biofilms formed by various microbial species, including gram-positive bacteria, gram-negative bacteria, and fungi such as Candida albicans.
Biofilms are medically significant because microorganisms adhering to surfaces produce extracellular polymers that facilitate adhesion and provide structural matrix, causing resistance to antimicrobial agents and detergents. The problem addressed is the need for long-term prevention of biofilm formation, effective antimicrobial compositions at low concentrations to reduce toxicity and side effects, medically acceptable and commercially economical compositions free of resistance that reduce biofilm formation on biomedical devices.
The compositions demonstrate enhanced antibiofilm activity when combined, allowing effective inhibition at lower concentrations than when compounds are used individually. The invention also relates to methods of disinfecting, coating, or impregnating surfaces and medical devices to prevent microbial biofilms, including use on wound care devices and treatment of wounds, as well as incorporation into various materials.
Claims Coverage
The patent includes a set of independent claims focused on antimicrobial compositions and methods for removing or reducing microbial biofilms and disinfecting surfaces.
Composition comprising sodium meta-periodate and chlorhexidine
A composition for removing or reducing microbial biofilms caused by Pseudomonas aeruginosa, Staphylococcus epidermis, and Escherichia coli, comprising (a) sodium meta-periodate at about 20 μg/mL to about 2000 μg/mL and (b) chlorhexidine base or salts at about 1 μg/mL to about 100 μg/mL.
Method of disinfecting surfaces with composition and disinfectant
A method to reduce or remove microbial biofilms caused by specified bacteria by disinfecting, cleaning, or rinsing a surface through contacting the surface with a combination of the composition comprising sodium meta-periodate and chlorhexidine and a disinfectant.
Method of disinfecting wounds using the composition
A method of disinfecting a wound comprising administering the composition containing sodium meta-periodate and chlorhexidine to the wound.
Method of spraying, dipping, overlaying, or coating objects with the composition
A method of treating an object to be disinfected by contacting it with the composition comprising sodium meta-periodate and chlorhexidine, applicable to diverse objects including dental devices, household items, industrial equipment, and medical instruments.
The claims focus on compositions comprising sodium meta-periodate and chlorhexidine with defined concentration ranges, and methods of using these compositions for disinfecting biofilms on medical, household, industrial, and other surfaces, including wounds, highlighting versatile antimicrobial applications.
Stated Advantages
Effective antimicrobial activity against biofilms formed by various gram-positive and gram-negative bacteria and fungi at lower concentrations of active compounds, reducing toxicity and side effects.
Enhanced antibiofilm activity when active compounds are combined, leading to more effective inhibition than individual compounds used alone.
Medically acceptable and relatively economical to manufacture on a commercial scale.
Reduced likelihood of developing bacterial resistance due to effective low concentration use.
Documented Applications
Disinfection and prevention of biofilms on medical devices such as indwelling catheters, urinary devices, wound drain tubes, endotracheal tubes, feeding tubes, dental instruments, surgical instruments, and wound care devices.
Treatment, cleaning, or disinfecting of wounds including chronic wounds, acute wounds, surgical wounds, burns, ulcers, and lacerations by administering the compositions or using wound care devices impregnated or coated with the compositions.
Coating or impregnating surfaces of household and industrial items including dental floss, dentures, mouth guards, dairy lines and filters, water lines, food and beverage manufacturing lines, general household disinfectants, laundry detergents, cleaning supplies, HVAC systems, vacuum cleaners, paint, wall coverings, filters, toys, cosmetic containers, plumbing fixtures, bathroom tiles, sinks, showers, toilets, sealants, towels, kitchen utensils, bathtubs, swimming pools, ponds, garden hoses, and hot tubs.
Cleaning and biofilm control in industrial pipelines such as dairy lines, food and beverage processing lines, paper mills, cooling towers, gas and oil pipelines, water treatment facilities, mining leaching processes, and preventing corrosion caused by organisms.
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