Purple transgenic fluorescent ornamental fish
Inventors
Blake, Alan • Crockett, Richard • Nasevicius, Aidas
Assignees
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Publication Number
US-8581023-B2
Publication Date
2013-11-12
Expiration Date
Abstract
The present invention relates to the method and use of fluorescent proteins in making purple transgenic fluorescent fish. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
Core Innovation
The invention relates to purple fluorescent transgenic ornamental fish that comprise recombinant constructs and transgenic fluorescent zebrafish. The fluorescent fish include chromosomally integrated FP635 fluorescent protein expression cassettes, and the zebrafish line is associated with the Purple zebrafish 1 transformation event, where sperm comprising the transformation event is deposited as ECACC accession no. 11012801.
The invention further provides fluorescent progeny fish that both exhibit fluorescence and comprise the Purple zebrafish 1 transformation event, including fertile transgenic fluorescent zebrafish. Golden zebrafish pigmentation/lightness is discussed as an ornamental line background through the slc24a5 mutation associated with melanosomes, in order to support purple fluorescence visibility in ornamental fish.
The described utilities include using fluorescent labeling as a research tool, including embryonic cell lineage and cell migration tracing, genetic mosaic experiments for cell marking, and fish cancer models. The document also describes providing Purple zebrafish 1 and fluorescent progeny fish to the ornamental fish industry through obtaining transgenic fluorescent progeny fish and distributing them to ornamental fish market channels.
Claims Coverage
The independent claims cover two inventive features: a fluorescent progeny fish and a method of producing a transgenic fluorescent fish. The coverage centers on chromosomally integrated FP635 expression cassettes tied to the Purple zebrafish 1 transformation event defined by ECACC accession no. 11012801, and on breeding to obtain progeny comprising the transformation event.
Fluorescent progeny fish with chromosomally integrated FP635 tied to Purple zebrafish 1
A fluorescent progeny fish of a transgenic fluorescent zebrafish that comprises a chromosomally integrated expression cassette encoding an FP635 fluorescent protein, wherein the zebrafish and progeny both exhibit fluorescence and comprise the Purple zebrafish 1 transformation event, sperm comprising the Purple zebrafish 1 transformation event having been deposited as ECACC accession no. 11012801.
Method producing transgenic fluorescent zebrafish progeny with Purple zebrafish 1
A method of producing a transgenic fish that exhibits fluorescence comprising obtaining a transgenic fluorescent zebrafish that exhibits fluorescence and comprises a chromosomally integrated expression cassette encoding an FP635 fluorescent protein, wherein the zebrafish comprises the Purple zebrafish 1 transformation event, and breeding the obtained zebrafish with a second fish to provide a transgenic fluorescent progeny fish comprising the Purple zebrafish 1 transformation event.
Overall, the claim set specifies fluorescent zebrafish and fluorescent progeny fish characterized by chromosomally integrated FP635 expression cassettes linked to the Purple zebrafish 1 transformation event defined by ECACC accession no. 11012801, and it covers producing such progeny by obtaining the transformation event-bearing zebrafish and breeding to obtain progeny comprising the transformation event.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Embryonic cell lineage and cell migration tracing using fluorescent labeling.
Cell marking for genetic mosaic experiments.
Fish cancer models.
Providing and distributing fluorescent fish to the ornamental fish industry/ornamental fish market channels, including grower/commercial distributor/retailer with an ornamental fish department.
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