Device for serologically detecting Yersinia infections and/or secondary diseases thereof and use of the proteins MyfA and PsaA of Y. enterocolitica and Y. pseudotuberculosis as recombinant antigens
Inventors
Assignees
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Publication Number
US-8580521-B2
Publication Date
2013-11-12
Expiration Date
Abstract
Devices are disclosed for serologically detecting an infection with human-pathogenic Yersinia ssp, wherein said device comprises at least one antigen selected from the group of antigens consisting of the following group: YopD, YopH, YopM, YopE, V-AG and YopN or a fragment of one of said antigens having at least eight consecutive amino acids and furthermore one of two proteins selected from MyfA and PsaA or fragments of one of said two proteins having at least eight consecutive amino acids.
Core Innovation
The invention relates to a device for serologically differentiating an infection with Yersinia enterocolitica from an infection with Yersinia pseudotuberculosis. The device comprises at least one antigen of a first group selected from YopD, YopH, YopM, YopE, V-AG, YopN and fragments thereof, and at least one antigen of a second group selected from MyfA, PsaA and fragments thereof. Each individual antigen from the first and second groups is fixed to the device in a spatially separated manner.
The disclosed approach addresses serological differentiation despite protein homology by using recombinant antigens and fragments that provide diagnostically relevant epitopes. The fragment embodiments have at least eight consecutive amino acids and at least one diagnostically relevant epitope. The documentation describes seroreactivity and species-separating patterns using line assays and a bead-based Luminex system, including IgG reactivity patterns to PsaA-MIK and MyfA-MIK within tested sample sets.
The diagnostic interpretation is based on positive reactions associated with YopD or other appropriate first-group antigen signals versus MyfA or PsaA second-group signals for assignment of infection with Yersinia enterocolitica or Yersinia pseudotuberculosis. Spatial separation of individually fixed first-group and second-group antigens on the device enables distinct readouts from the same serological sample.
Claims Coverage
The document includes two independent claims directed to a serological differentiation device and a method of preparing such a device. The coverage centers on two antigen groups with defined antigen identities and fragment constraints, together with spatial separation on the device.
Spatially separated immobilized two-group Yersinia antigens
A device comprising at least one first group antigen selected from YopD, YopH, YopM, YopE, V-AG, YopN and fragments thereof having at least eight consecutive amino acids and at least one diagnostically relevant epitope, and at least one second group antigen selected from MyfA, PsaA, and fragments thereof having at least eight consecutive amino acids and at least one diagnostically relevant epitope, wherein each individual first and second group antigen is fixed to the device in a spatially separated manner.
Preparing a spatially separated two-group antigen device
A method of preparing a device for serologically differentiating an infection caused by Yersinia enterocolitica from an infection caused by Yersinia pseudotuberculosis, comprising providing at least one second group antigen selected from MyfA, PsaA and fragments thereof comprising at least 8 consecutive amino acids and at least one diagnostically relevant epitope; providing at least one first group antigen selected from YopD, YopH, YopM, YopE, V-AG, YopN and fragments thereof having at least 8 consecutive amino acids and at least one diagnostically relevant epitope; and fixing each of said first and second group antigens to the device in a spatially separated manner.
Overall, the claim set requires two distinct antigen groups with defined antigen identities and fragment constraints and requires spatially separated immobilization of individually fixed first-group and second-group antigens on the device. The method claim corresponds to preparing a device that meets these structural requirements.
Stated Advantages
The device enables serological differentiation of an infection with Yersinia enterocolitica from an infection with Yersinia pseudotuberculosis.
Spatial separation of the individually fixed antigens enables distinct readouts from the same serological sample.
The use of recombinant antigens and fragments provides diagnostically relevant epitopes despite protein homology.
Documented Applications
Serological differentiation of Yersinia enterocolitica infection from Yersinia pseudotuberculosis infection.
Line assays.
Bead-based Luminex system.
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