Combined effects of nutrients on proliferation of stem cells
Inventors
Davis Sanberg, Cyndy • Bickford, Paula • Sanberg, Paul • Tan, Jun • Shytle, R. Douglas
Assignees
US Department of Veterans Affairs • Natura Therapeutics Inc • University of South Florida St Petersburg
Publication Number
US-8574640-B2
Publication Date
2013-11-05
Expiration Date
2026-05-02
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Abstract
A method and composition for stimulating the proliferation and differentiation of stem cells is used to self-repair injury in mammals. A supplement is administered having an effective dose of blueberry, carnosine, catechin, green tea extract, VitaBlue, Vitamin D3 or combinations of these. For example, a therapeutic amount of two or more of the supplements may be selected having a synergistic effect, allowing a lower dose to achieve the same or greater effective protection as a higher dose of any one of the supplements.
Core Innovation
The invention provides a method and compositions for stimulating proliferation and differentiation of stem cells to promote self-repair of injury in mammals. The method involves administering a supplement containing effective doses of natural substances such as blueberry extract, carnosine, catechin, green tea extract, VitaBlue, and Vitamin D3, either alone or in synergistic combinations. These combinations allow for smaller doses to achieve the same or greater efficacy in protecting and repairing tissues compared to higher doses of individual supplements.
The problem addressed by this invention arises from the reduced proliferative activity of endogenous stem cells in adult mammals, particularly in aging and age-associated diseases like cardiovascular disease, stroke, Parkinson's disease, and other conditions. Existing treatments that stimulate stem cell proliferation, like cytokine therapies, are costly and entail certain risks. Moreover, the amounts of natural substances needed to obtain health benefits from natural products are often impractical to consume in ordinary diets.
The invention solves this problem by identifying specific natural compounds and demonstrating their synergistic effects in promoting stem cell proliferation in vitro and in vivo. The combination of blueberry extract, green tea extract, carnosine, and Vitamin D3 was shown to synergistically increase cell proliferation of human bone marrow cells, hematopoietic progenitors (CD34+ cells), and peripheral blood-derived progenitor cells (CD133+ cells). These combinations yielded proliferation effects equal to or exceeding those of the cytokine hGM-CSF while enabling administration at practical doses suitable for dietary supplementation.
Claims Coverage
The patent includes two main independent claims covering novel compositions and methods for stimulating stem cell proliferation.
Composition for stimulation of stem cell proliferation
A composition comprising 400 mg to 25 grams of blueberry extract, 10 mg to 100 mg of carnosine, and 400 mg to 5 grams of green tea extract to promote stem cell proliferation.
Method for increasing stem cell proliferation
Oral administration of a composition comprising 400 mg to 25 grams of blueberry extract, 10 mg to about 100 mg of carnosine, and about 400 mg to 5 grams of green tea extract to a human to increase stem cell proliferation.
The independent claims focus on compositions combining specific ranges of blueberry extract, carnosine, and green tea extract to stimulate stem cell proliferation, and methods involving oral administration of these compositions to humans. Additional claims include optional inclusion of vitamin D3 to enhance effectiveness.
Stated Advantages
Synergistic effects of combined natural substances allow lower doses to achieve equal or greater stem cell proliferation compared to single agents.
Effective promotion of proliferation and differentiation of multiple stem and progenitor cell types, including bone marrow, CD34+, and CD133+ cells.
Potential for natural, dietary-based therapies to promote self-repair in mammals with fewer risks and costs than cytokine treatments.
Demonstrated neuroprotective effects in animal models of stroke, including improved behavioral and histological outcomes.
The compositions do not reduce bone marrow progenitor counts with long-term administration, unlike certain cytokine therapies.
Documented Applications
Treatment and repair of aging-associated declines in stem cell proliferation in diseases such as cancer, diabetes, stroke, amyotrophic lateral sclerosis (ALS), Parkinson's disease, and cardiovascular disease.
Dietary supplementation to promote stem cell proliferation, differentiation, and migration for endogenous tissue repair following injury or in disease states.
Neuroprotection and functional improvement in induced stroke in mammals via enhanced neural stem cell proliferation and differentiation.
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