Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-8563329-B2
Publication Date
2013-10-22
Expiration Date
Abstract
Modified branched polymers are combined with bioactive agents which are one member of a binding pair for use in an assay.
Core Innovation
The invention relates to detecting a target in a sample using an assay kit that includes a detector that specifically binds the target, a conjugate that comprises a modified dendritic polymer and a second member of a binding pair, a capture molecule that comprises a member of a second binding pair which specifically binds the target, and a reporter. The detector is tagged with a plurality of a first member of the first binding pair, and the conjugate couples the modified dendritic polymer with the second member of the first binding pair so that the binding pair components participate in target recognition and signal generation.
The assay kit combines a specific-binding detector/capture arrangement with a polymer-based conjugate built from modified dendritic polymer. The modified dendritic polymer is used as the conjugate platform, and the binding pair members connect the polymer conjugate to the detector/capture interactions associated with the target. The kit further provides a reporter to generate an assay signal corresponding to target detection.
Claims Coverage
The provided independent claim defines an assay kit with four core components: detector, polymer-based conjugate, capture molecule, and reporter. The claim also specifies that the detector is tagged with multiple members of a first binding pair while the conjugate comprises a modified dendritic polymer and a second member of the binding pair.
Assay kit with detector, modified dendritic polymer conjugate, capture molecule, and reporter
An assay kit for detecting a target in a sample, comprising a detector that specifically binds the target and is tagged with a plurality of a first member of a first binding pair, a conjugate comprising a modified dendritic polymer and a second member of the first binding pair, a capture molecule comprising a member of a second binding pair which specifically binds the target, and a reporter.
Detector tagged with multiple members of a binding pair
The detector specifically binds the target and is tagged with a plurality of a first member of a first binding pair.
Modified dendritic polymer conjugate with binding-pair member
A conjugate comprising a modified dendritic polymer and a second member of the first binding pair.
Capture molecule with second binding-pair member that binds the target
A capture molecule comprising a member of a second binding pair which specifically binds the target.
Reporter for assay readout
A reporter as part of the assay kit for detecting the target in the sample.
The main inventive structure is an assay kit architecture that links a specifically binding detector tagged with multiple binding-pair members, a conjugate formed from a modified dendritic polymer and a binding-pair member, a capture molecule carrying a complementary binding-pair member, and a reporter.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
Interested in licensing this patent?