Peptides and compositions for prevention of cell adhesion and methods of using same

Inventors

Zlotkin, Amir

Assignees

Dispersebio Ltd

Abstract

Compositions comprising an isolated peptide, which may for example optionally comprise a sequence selected from the group consisting of FDYDWY (SEQ ID NO: 2), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3) or CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1), or any cyclized version thereof, and methods of using same, including for treatment of or prevention of formation of microbial biofilms and against adhesion of a cell to a surface.

Core Innovation

The invention relates to isolated anti-adhesive cyclized peptides comprising a length of up to 35 amino acids and comprising a sequence selected from FDYDWY (SEQ ID NO: 2), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3), and CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1). The peptides are cyclized and are used to prevent cell adhesion and microbial substrate adhesion.

The problem addressed is biofilm formation and adhesion of microbial single cell organisms on substrates, including medical devices and other surfaces, which is linked to infections arising from biofilm formation. The disclosed cyclized peptides are characterized as lacking bactericidal and growth-inhibitory activity, focusing on anti-adherence rather than killing or growth suppression.

The peptides are documented as anti-biofilm and anti-adhesion agents that reduce attachment of single cell organisms to a substrate and thereby reduce biofilm formation and biofouling. The disclosure further supports use on surfaces and devices, including treatment contexts intended to prevent adhesion and fouling of filters and other substrates.

Claims Coverage

The claims are grounded around cyclized peptides of up to 35 amino acids that comprise specific sequence selections, with further claim sets directed to compositions and methods that inhibit adhesion/biofilm formation by contacting single cell organisms or treated media with a surface. Across the independent claims, three main inventive areas are covered: the peptide structure itself, a composition containing that peptide, and an adhesion-inhibition method that contacts single cell organisms or treated media with a surface.

Overall claim coverage is directed to defined cyclized peptides (up to 35 amino acids) comprising specific anti-adhesive sequence selections, to compositions comprising those peptides, and to methods that inhibit adhesion of single cell organisms to a surface by contacting the cell with the claimed composition, including downstream contexts such as water/fluid treatment for reducing biofilm formation and filter fouling, and reverse osmosis filter use in dependent claims.

Stated Advantages

Documented Applications