Method for treating skin wounds
Inventors
Gogly, Bruno • Coulomb, Bernard • Lafont, Antoine
Assignees
L'ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS - 333% PART INTEREST • Assistance Publique Hopitaux de Paris APHP • Institut National de la Sante et de la Recherche Medicale INSERM • Inserm Transfert SA • Universite Paris Cite
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Publication Number
US-8303948-B2
Publication Date
2012-11-06
Expiration Date
Abstract
The present invention relates to a method for treating a skin wound in an individual, comprising applying to the skin wound of the individual a therapeutically effective quantity of gingival fibroblasts.
Core Innovation
The invention relates to a method of accelerating or improving healing of a skin wound in an individual by applying to the skin wound of the individual a quantity of isolated gingival fibroblasts. The described approach positions gingival fibroblasts as therapeutic cells for wound healing, applied directly to the skin wound.
The method addresses the treatment of skin wounds and may further include keratinocytes and/or wound-healing activating compounds. The gingival fibroblasts are presented as an alternative to dermal fibroblasts, with gingival fibroblasts described as especially suitable due to availability, especially for large burns, and a higher expansion capacity.
The description further characterizes application formats for the gingival fibroblasts, including liquid or gel cell suspensions, sprays, and gingival fibroblast cultures grown on biocompatible and preferably bioresorbable collagen lattices as dermal equivalents. The collagen lattices are described as 3D collagen lattices, including collagen type I lattices, configured to support dermal equivalent and tissue remodeling outcomes.
The described preliminary results indicate that the gingival fibroblasts promote tissue remodeling and epidermal growth and differentiation, and promote neo-dermis formation in a rat wound model, compared to dermal fibroblasts. The description characterizes these outcomes as dermal formation, tissue remodeling, epidermalization promotion, and in vivo wound healing effects.
Claims Coverage
The claims cover one independent method claim, plus dependent claim refinements that specify the fibroblast source and a particular application format. The core inventive coverage centers on applying isolated gingival fibroblasts to the skin wound.
Applying isolated gingival fibroblasts to the skin wound
Accelerating or improving healing of a skin wound in an individual by applying to the skin wound of the individual a quantity of isolated gingival fibroblasts.
Autologous gingival fibroblasts derived from the individual
The method wherein the gingival fibroblasts are derived from the individual.
Gingival fibroblast culture on a biocompatible lattice as a dermal equivalent
The method wherein the gingival fibroblasts are applied to a skin wound as a culture grown on a biocompatible lattice.
Overall, the claim set focuses on treating skin wounds in an individual by applying isolated gingival fibroblasts, optionally refined to autologous fibroblasts and applied as a culture grown on a biocompatible lattice.
Stated Advantages
Availability of gingival fibroblasts, especially for large burns.
Higher expansion capacity compared with dermal fibroblasts.
Ability to promote dermal formation and tissue remodeling.
Promotion of epidermal growth and differentiation.
Promotion of neo-dermis formation in vivo.
Documented Applications
Treating skin wounds in an individual by applying isolated gingival fibroblasts to the skin wound.
Using gingival fibroblasts as an alternative to dermal fibroblasts for wound healing.
Application in formats including liquid or gel cell suspensions, sprays, and gingival fibroblast cultures on biocompatible, preferably bioresorbable collagen lattices as dermal equivalents.
Rat wound model context showing preliminary in vivo wound healing, tissue remodeling, epidermalization promotion, and neo-dermis formation.
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