Vaccine for Edwardsiella disease and streptococcal disease in fish
Inventors
Takahashi, Yukinori • Abe, Michinari
Assignees
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Publication Number
US-8246963-B2
Publication Date
2012-08-21
Expiration Date
Abstract
Disclosed is a vaccine for edwardsiellosis and streptococcosis in a fish. Specifically, disclosed is a vaccine for edwardsiellosis in a fish, which comprises inactivated cells of (A) an Edwardsiella tarda strain derived from the target fish, and inactivated cells of (B) an Edwardsiella tarda strain derived from a fish other than the target fish, wherein, when the strain (A) is a typical Edwardsiella tarda strain, the strain (B) is an atypical Edwardsiella tarda strain, whereas when the strain (A) is an atypical Edwardsiella tarda strain, the strain (B) is a typical Edwardsiella tarda strain. Also specifically disclosed is a vaccine for edwardsiellosis and/or streptococcosis in a fish, which comprises the components mentioned above and inactivated cells of (C) Streptococcus iniae and/or Streptococcus parauberis.
Core Innovation
The invention relates to a vaccine for edwardsiellosis in a target fish, specifically a bastard halibut or a red sea bream. The vaccine comprises inactivated Edwardsiella tarda cells from a strain obtained from the target fish and inactivated Edwardsiella tarda cells from a strain obtained from a fish other than the target fish, with typical and atypical Edwardsiella tarda strains assigned in a reciprocal manner.
When the strain obtained from the target fish is a typical Edwardsiella tarda strain, the strain obtained from the other fish is an atypical Edwardsiella tarda strain, and when the strain obtained from the target fish is an atypical Edwardsiella tarda strain, the strain obtained from the other fish is a typical Edwardsiella tarda strain. When the target fish is a bastard halibut, the fish other than the target fish is a red sea bream, and when the target fish is a red sea bream, the fish other than the target fish is a bastard halibut.
The co-presence of inactivated E. tarda derived from the target fish and from another fish species is associated with activation of phagocyte bactericidal capacity with lymphocytes, enhanced leukocyte phagocytosis, and macrophage rapid killing. The described vaccine can further include inactivated Streptococcus iniae and/or Streptococcus parauberis for protection against mixed infections.
Claims Coverage
The independent claim is directed to a vaccine for edwardsiellosis in a target fish comprising inactivated Edwardsiella tarda cells from the target fish and from another fish species, with typical/atypical strains assigned in a reciprocal manner. Three additional inventive features are specified in the claim set.
Reciprocal typical and atypical inactivated Edwardsiella tarda from target and other fish species
A vaccine for edwardsiellosis in a target fish comprising inactivated cells of an Edwardsiella tarda strain obtained from the target fish and inactivated cells of an Edwardsiella tarda strain obtained from a fish other than the target fish, wherein typical and atypical Edwardsiella tarda strains are assigned reciprocally.
Target fish species determines the other fish species pairing
When the target fish is a bastard halibut, the fish other than the target fish is a red sea bream, and when the target fish is a red sea bream, the fish other than the target fish is a bastard halibut.
Injection or oral administration
The vaccine is administered by injection or by oral administration.
The claim coverage centers on a two-strain inactivated Edwardsiella tarda vaccine using reciprocal typical/atypical assignment between the target fish and another fish species, with the bastard halibut/red sea bream pairing. The claim set also includes administration by injection or oral administration.
Stated Advantages
Enhanced leukocyte phagocytosis.
Macrophage rapid killing.
Improved survival after Edwardsiella tarda challenge in bastard halibut and sea bream using the A/B mixed-strain vaccine versus single-strain controls.
Effectiveness under injection and oral administration.
Improved prevention of both edwardsiellosis and streptococcosis when adding inactivated Streptococcus iniae and/or Streptococcus parauberis.
Documented Applications
Protection against edwardsiellosis in a target fish that is a bastard halibut or a red sea bream.
Protection against mixed infections by combining the edwardsiellosis vaccine with inactivated Streptococcus iniae and/or Streptococcus parauberis, including prevention of both edwardsiellosis and streptococcosis.
Edwardsiella tarda challenge experiments in bastard halibut and sea bream showing improved survival with the mixed-strain vaccine.
Use in both injection administration and oral administration formats.
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