Inbred corn line NPID4466
Inventors
Assignees
Syngenta Crop Protection AG Switzerland • National Aeronautics and Space Administration NASA
Publication Number
US-8017843-B1
Publication Date
2011-09-13
Expiration Date
2029-02-26
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Abstract
Basically, this invention provides for an inbred corn line designated NPID4466, methods for producing a corn plant by crossing plants of the inbred line NPID4466 with plants of another corn plant. The invention relates to the various parts of inbred NPID4466 including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line NPID4466 and plants produced by said methods.
Core Innovation
The invention provides an inbred corn line designated NPID4466, including the seeds, plants, and various plant parts derived from this line. The inbred corn line NPID4466 can be used to produce hybrid seed by crossing with other corn lines, and methods for producing hybrid corn plants from such crosses are also described. The invention includes tissue culture of regenerable cells of NPID4466 that can regenerate plants expressing its genotype and phenotype, as well as mutants or variants thereof.
This invention addresses the challenge that inbred corn lines, while homozygous and uniform, suffer from reduced vigor, making seed production difficult. Crossing two inbreds produces heterozygous hybrid plants that are more robust and have increased seed yield. The key problem being solved is developing an inbred corn line with desirable physiological and morphological characteristics that can be reliably used to produce high yielding, agronomically sound hybrid corn adapted for various regions.
Further, the invention covers methods of introducing one or more targeted traits, including transgenic and non-transgenic traits, into the NPID4466 inbred to produce backcrossed progeny maintaining the physiological and morphological characteristics of NPID4466. This includes traits such as male sterility, herbicide resistance, insect resistance, disease resistance, and modified starch traits. The invention also encompasses the use of molecular markers to accelerate breeding and trait introgression into the inbred.
Claims Coverage
The claims define multiple inventive features focused on the inbred corn line NPID4466, plants, seeds, hybrid production, and methods of introducing and incorporating desired traits.
Inbred corn seed and plants
Claims cover the seed of maize variety NPID4466 and maize plants grown therefrom, including plant parts and tissue culture cells regenerable from these plants.
F1 hybrid seed and plants produced by crossing NPID4466
Claims include F1 hybrid maize seed produced by crossing plants of NPID4466 with different maize varieties, and the plants or parts grown from such hybrid seed.
Methods of producing inbred and hybrid seed
Claims cover methods of producing maize seed by crossing NPID4466 plants with themselves or with other maize plants, including selfing and hybrid seed production methods.
Methods for introducing desired traits into NPID4466
Claims disclose a method of introducing a desired trait into NPID4466 by crossing with donor plants comprising traits such as waxy starch, amylase, male sterility, herbicide resistance, insect resistance, and disease resistance, followed by selection and backcrossing to recover plants with the trait and NPID4466 characteristics.
Transgene introgression and trait incorporation
Claims include methods of producing NPID4466 plants with added traits conferred by transgenes or trait alleles, including herbicide resistance to specific herbicide classes and other traits like insect tolerance, pathogen resistance, and modified carbohydrate or fatty acid metabolism.
Methods of producing plant products from hybrids
Claims cover producing plant products such as silage, starch, ethanol, oil, syrup, amylase, and protein from plants grown from hybrids involving NPID4466.
Breeding methods to develop derived plants
Claims include methods of developing maize derived plants from NPID4466 by crossing, breeding techniques including recurrent selection, backcrossing, pedigree breeding, and marker assisted selection.
The claims collectively cover the inbred corn line NPID4466 and its seeds and plants, the production of hybrid seeds and plants derived therefrom, and detailed methods for introducing desired traits into NPID4466 while retaining its characteristics. They further cover the use of tissue culture, transgenic modifications, and various breeding methods to produce improved corn plants and products based upon this inbred.
Stated Advantages
The inbred line NPID4466 produces uniform and stable plants with desirable physiological and morphological characteristics suitable for use in hybrid production.
Hybrid plants derived from NPID4466 exhibit increased vigor and seed yield compared to inbred plants.
Use of NPID4466 facilitates production of high yielding, agronomically sound hybrids adapted to various regions of the Corn Belt.
Incorporation of targeted traits and transgenes into NPID4466 can be efficiently achieved while maintaining the original line's agronomic performance, particularly using marker assisted breeding.
Documented Applications
Production of hybrid corn seed and hybrid corn plants by crossing NPID4466 with other inbred corn lines.
Use in methods for introgressing targeted traits such as male sterility, herbicide resistance, insect resistance, disease resistance, and starch modification into corn inbred lines.
Use of regenerable tissue culture cells of NPID4466 for plant regeneration and breeding.
Production of agricultural and industrial products including human food, livestock feed, starch, ethanol, oil, syrup, meal, amylase, and protein derived from plants bred from NPID4466 or its hybrids.
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