Cell strain capable of being cultured without ingredients derived from animals, method of producing the same, method of producing virus using the same, and method of producing vaccine
Inventors
Assignees
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Publication Number
US-7910366-B2
Publication Date
2011-03-22
Expiration Date
Abstract
The invention relates to a cell strain induced from MDCK cells as dog kidney-derived cells, and being able to be cultured without ingredients derived from animals. The cell strain is produced by adapting a MDCK cell to a medium without a serum but with a cell growth factor; and culturing the cell in a medium with an RPMI 1640 medium and a soybean-derived peptone but without ingredients derived from animals.
Core Innovation
A serum-free culture-adapted MDCK-derived dog kidney cell strain is provided and is deposited under accession No. FERM BP-10225. The strain is characterized as an MDCK-derived dog kidney-derived strain adapted to serum-free culture without animal-derived ingredients, and it supports virus production by infecting and culturing infected cells to replicate virus.
The culture adaptation transitions to a defined medium for serum-free culture. A serum-free medium containing a cell growth factor is used, and the infected cell culture is described with RPMI 1640 medium and soybean-derived peptone as RPMI/SP medium. The medium composition lacks animal-derived ingredients, and a cryopreservation medium is described as RPMI/SP plus 10% DMSO.
Virus production is described as achievable using monolayer culture and suspension culture, including suspension and microcarrier beads. The document reports virus infectivity titers that can be comparable or improved versus conventional bovine-serum culture for multiple canine viruses, including canine distemper virus, canine adenovirus type 1 and type 2, canine parainfluenza virus, and canine parvovirus type 2.
Claims Coverage
Independent claim clm-00001 covers an isolated deposited cell strain deposited under accession No. FERM BP-10225. The claim set also describes virus production using the deposited strain, with medium composition restrictions excluding animal-derived ingredients, suspension culture, and defined virus selections.
Isolated cell strain deposited under accession No. FERM BP-10225
An isolated cell strain comprising a cell strain deposited under accession No. FERM BP-10225.
Virus production by infecting and culturing the deposited cell strain
A method of producing a virus by infecting the deposited cell strain and culturing the infected cell strain to replicate the virus.
Serum-free medium using RPMI 1640 with soybean-derived peptone and excluding animal-derived ingredients
The virus-production method wherein the culture medium includes RPMI 1640 and soybean-derived peptone but excludes ingredients derived from animals.
Suspension culture method for culturing infected cells
The virus-production method wherein culturing the infected cell strain is performed by a suspension culture method.
Defined virus families for the virus-production method
The virus-production method wherein the virus is selected from the group consisting of Paramyxoviridae, Orthomyxoviridae, Rhabdoviridae, Flaviviridae, Caliciviridae, Adenoviridae, Herpesviridae, and Parvoviridae.
Defined virus types within specific canine and related viruses
The virus-production method wherein the virus is selected from the group consisting of canine distemper virus, measles virus, canine parainfluenza virus, SV5 virus, influenza virus, rabies virus, Japanese encephalitis virus, canine calicivirus, canine adenovirus type 1 and type 2, human adenovirus, canine herpesvirus, and canine parvovirus type 1 and type 2.
The claim coverage centers on the isolated MDCK-derived dog kidney cell strain deposited under FERM BP-10225 and its use for virus production by infecting and culturing to replicate virus. The dependent features emphasize serum-free, animal-derived-ingredient-excluding medium composition using RPMI 1640 and soybean-derived peptone, suspension culture, and defined selections of virus families and particular virus types.
Stated Advantages
Virus infectivity titers can be comparable or improved versus conventional bovine-serum culture for multiple canine viruses.
Documented Applications
Vaccine production, including inactivated vaccine and live/attenuated vaccine.
Production of diagnostic antigen, including ELISA using diagnostic reagents described in the document.
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