analyzing a sample comprising directing a beam of light to a spot on the surface of a microarray treated with the sample using a digital micromirror device, and observing the surface plasmon resonance (SPR) spectral shift due to a chemical binding event
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Publication Number
US-7745143-B2
Publication Date
2010-06-29
Expiration Date
Abstract
A method of analyzing a sample comprising directing a beam of light to a spot on the surface of a microarray treated with the sample using a digital micromirror device, and observing the SPR spectral shift due to a chemical binding event. The digital micromirror device can be, for example, that used in Digital Light Processing (DLP). Such a device can selectively place a pixel of light onto a microarray such that each spot can be observed at millisecond intervals and the whole microarray can be sequentially scanned over a relatively short period. The SPR spectral shift for each spot can be measured as a function of time, thus producing SPR detection of molecular binding in an array format.
Core Innovation
The invention provides a method for analyzing a sample using an SPR microarray in an array format. A microarray includes a surface having a plurality of discrete locations, where each discrete location includes a first member of a chemical binding pair associated with a surface plasmon resonance. When the microarray surface is contacted with a sample having a second member of the chemical binding pair, binding occurs at at least one discrete location and causes an SPR spectral shift at the discrete location where the chemical binding occurs.
The method irradiates the discrete locations with a light beam using an apparatus that includes a light source, a digital micromirror device, and a prism or lens located between the digital micromirror device and the microarray surface. The invention uses selective illumination of discrete microarray spots and detects SPR spectral shift for each location as a function of time.
An SPR microarray reader hardware architecture is described, including a light source, a digital micromirror device, prism or lens coupling optics, optics for non-imaged or spatially discriminated reflected radiation, a spectrometer, and a computer control system. Time-resolved SPR detection produces array format surface plasmon resonance detection of chemical binding with the sample by generating binding curves for chemical binding events.
Claims Coverage
The document includes one independent claim covering an SPR microarray sample analysis method, with the core inventive combination of a microarray with discrete SPR-associated locations and chemical binding pairs, selective irradiation using a digital micromirror device with prism or lens coupling, and time-dependent detection of SPR spectral shift for each location in an array format.
Microarray discrete SPR locations with chemical binding pair spectral shift
Providing a microarray with a plurality of discrete locations, each including a first member of a chemical binding pair associated with a surface plasmon resonance, where contacting with a sample having a second member binds at a discrete location and causes a spectral shift in the surface plasmon resonance associated with that discrete location.
Digital micromirror controlled illumination with prism or lens
Irradiating the discrete locations on the microarray surface with a light beam using an apparatus comprising a light source, a digital micromirror device, and a prism or lens located between the digital micromirror device and the microarray surface.
Time-resolved spectral shift detection for array format chemical binding
Detecting the surface plasmon resonance spectral shift associated with each location as a function of time to provide surface plasmon resonance detection of chemical binding with the sample in an array format.
The inventive concept is the combination of discrete SPR-associated microarray locations with chemical binding pair-induced SPR spectral shifts, selective irradiation of discrete locations using a digital micromirror device with prism or lens coupling, and time-resolved detection of SPR spectral shift per location to provide array format SPR detection of chemical binding.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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