NAD(P)- dependent responsive enzymes, electrodes and sensors, and methods for making and using the same
Inventors
Ouyang, Tianmei • Feldman, Benjamin J.
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-12698517-B2
Publication Date
2026-08-04
Expiration Date
Abstract
NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.
Core Innovation
The invention provides an electrochemical analyte sensor configured for partial insertion below a subject’s skin surface, having a portion positioned above the subject’s skin surface and an inserted portion positioned below the subject’s skin surface. In the inserted portion, a non-conductive material layer supports a first conductive layer configured as a working electrode.
An enzyme composition is disposed on a surface of the working electrode and includes NAD(P)+ or a derivative thereof, D-3-hydroxybutyrate dehydrogenase, diaphorase, and an electron transfer agent. The NAD(P)+ or derivative thereof is not covalently bonded to the polymer included in the enzyme composition.
A mass transport limiting membrane is disposed over the enzyme composition to limit flux of D-3-hydroxybutyrate to the working electrode. The disclosed sensor is further described as suitable for in vivo/transcutaneously positioned operation, including electrode architectures for working/reference/counter functions and optional diffusion- or mass-transport limiting membrane layers.
Claims Coverage
Independent claim clm-00001 includes four inventive features.
Partially inserted electrochemical analyte sensor
An electrochemical analyte sensor configured for partial insertion below a subject's skin surface, with a portion above the skin surface and an inserted portion below the skin surface.
Working electrode with enzyme composition
An inserted portion comprising a non-conductive material layer and a first conductive layer disposed on the non-conductive material layer, where the first conductive layer is a working electrode and an enzyme composition is disposed on a surface of the working electrode.
Enzyme composition with NAD(P)+ not covalently bound and D-3-hydroxybutyrate dehydrogenase
The enzyme composition comprises NAD(P)+ or derivative thereof (not covalently bonded to the polymer), D-3-hydroxybutyrate dehydrogenase, diaphorase, and an electron transfer agent, and further includes a polymer within the enzyme composition.
Mass transport limiting membrane for limiting D-3-hydroxybutyrate flux
A mass transport limiting membrane disposed over the enzyme composition to limit flux of D-3-hydroxybutyrate to the working electrode.
Across the independent claim, inventive coverage centers on a skin-insertable electrochemical sensor in which a working electrode carries an enzyme composition containing NAD(P)+ (not covalently bonded to a polymer), D-3-hydroxybutyrate dehydrogenase, diaphorase, and an electron transfer agent, with a mass transport limiting membrane over the enzyme composition configured to limit D-3-hydroxybutyrate flux to the working electrode.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
Interested in licensing this patent?