Marker assisted selection of traits for producing meal from Brassica napus
Inventors
Ehlert, Zoe Christina • Flook, Joshua A. • Garcia, Daniel • Knievel, Donna Carolynn • Ochsenfeld, Cherie • Patterson, Thomas G. • Preuss, Ryan • Rizvi, Syed Masood • Ripley, Van L. • Rounsley, Steve • Tang, Shunxue • Tahir, Muhammad • Wiggins, Michelle
Assignees
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Publication Number
US-12692555-B2
Publication Date
2026-07-28
Expiration Date
Abstract
A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.
Core Innovation
The disclosure describes fine mapping of quantitative trait loci (QTLs) for low fiber content (acid detergent fiber, ADF) and yellow seed coat (YSC) traits in Brassica napus using high-density SNP genotyping and multiple dihaploid populations derived from YN01-429. A major QTL on linkage group N09 explains approximately 59.2–74.7% of phenotypic variance across populations, and a minor QTL on N11 explains approximately 1.4–3.2%.
Seed coat traits (whiteness index WI and lightness index L) are correlated with ADF and co-localize with the QTLs. Within the N09 major QTL interval (0.0–4.9 cM), the disclosure identifies 18 SNP markers for marker-assisted selection, with flanking markers DBSNP01120 and DBSNP02172 defining a 4.9 cM interval.
The disclosure also specifies additional marker sub-intervals for YN01-429 tracking and describes conversion of QTL-associated SNP markers into assay-ready marker sets.
Claims Coverage
The relevant independent claim covers a breeding method of Brassica napus plants centered on an N09 chromosomal interval represented by the N09 chromosomal interval of line YN01-429 or YN-97-262, flanked by specific sequence identifiers, and uses marker assisted selection to select progeny or germplasm having lower ADF.
Breeding using an N09 chromosomal interval flanked by SEQ ID markers for lower ADF
Crossing a first Brassica napus plant with a second Brassica napus plant and thereby producing progeny, wherein the first plant comprises an N09 chromosomal interval of Brassica napus line YN01-429 or YN-97-262 flanked by SEQ ID NO:2 and SEQ ID NO:111; using marker assisted selection to analyze the progeny plants or germplasm thereof for the presence or the absence of the N09 chromosomal interval comprising a plurality of markers selected from SEQ ID NO:2 through SEQ ID NO:111; and selecting one or more progeny plants or germplasm thereof having the N09 chromosomal interval, wherein the selected progeny plants have lower ADF than the second Brassica napus plant.
The claim coverage is focused on marker-assisted breeding for lower ADF by selecting progeny or germplasm carrying an N09 chromosomal interval defined by specific flanking sequence identifiers and a plurality of markers from SEQ ID NO:2 through SEQ ID NO:111. Dependent claims further refine the plurality of markers and add downstream steps that use selected progeny to form plant lines, plant fields, grow plants carrying the interval, collect seed or grain, and produce meal.
Stated Advantages
Lower ADF in selected progeny plants having the N09 chromosomal interval.
Documented Applications
Breeding Brassica napus plants using marker assisted selection to select progeny or germplasm with an N09 chromosomal interval for lower ADF.
Plant line development from selected progeny plants having the N09 chromosomal interval.
Field planting with a Brassica napus plant line having the N09 chromosomal interval, growth of plants having the interval, and collection of seed or grain.
Producing meal from the collected seed or grain.
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