Analyte sensors employing multiple enzymes and methods associated therewith

Inventors

Oja, Stephen • Ouyang, Tianmei • Cho, Hyun • Tran, Lam N. • Feldman, Benjamin J.

Assignees

Abbott Diabetes Care Inc

Abstract

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

Core Innovation

The disclosure relates to a sensor control device for detecting glucose and ketones in vivo using a sensor that has a ketones-responsive active area and a glucose-responsive active area. A first working electrode carries the ketones-responsive active area and a second working electrode carries the glucose-responsive active area, with the electrodes electrically isolated from one another by dielectric material. A reference electrode is provided, and a mass transport limiting membrane overcoats the ketones-responsive active area and the glucose-responsive active area.

The mass transport limiting membrane provides variable analyte permeability at each of the ketones-responsive active area and the glucose-responsive active area, enabling distinct sensing behavior for different analyte regions. The membrane overcoating at least the ketones-responsive active area comprises a multi-component membrane, including bilayer or multi-component membrane approaches. The disclosure further emphasizes enabling multi-analyte sensing with independent sensitivity control via different membrane permeability behaviors for different electrode regions.

The sensor is configured to be partially inserted into a user's skin such that a distal portion of the sensor is in contact with interstitial fluid to detect glucose and ketones in vivo. Sensor electronics are included for operation of the sensor. The described embodiments support independent electrochemical readout using separated oxidation-reduction potentials and electrode configurations.

Claims Coverage

The partial content identifies one independent claim directed to a sensor control device for detecting glucose and ketones in vivo. The inventive features are centered on the sensor architecture, the spatially variable mass transport limiting membrane with multi-component construction, and in vivo placement with interstitial fluid contact.

Across the independent claim, the core claim coverage is achieved by combining an electrode architecture with dielectric isolation, a mass transport limiting membrane that provides variable analyte permeability for spatially distinct ketone and glucose active areas, and a multi-component membrane overcoating for at least the ketones-responsive region, in a device configured for in vivo interstitial fluid sensing with sensor electronics.

Stated Advantages

Documented Applications