Multiplex detection in high resolution devices through measurement of localized fluorescence ratios
Inventors
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-12584911-B2
Publication Date
2026-03-24
Expiration Date
Abstract
Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.
Core Innovation
A system and method detect one or more target analytes in a sample using a sample plate with a plurality of capture structures attached to the sample plate surface. Each capture structure independently comprises a detection portion configured to couple with a target analyte and a stem comprising a capture structure code, where the capture structure code comprises a plurality of flowers that each independently comprise a piece of nucleic acid or polymer coupled to at least one detectable molecule.
The detectable molecules embody the capture structure code, and the detectable molecules in combination uniquely identify a type of the capture structure associated with the detection portion. An excitation source generates excitation energy delivered into the sample plate, and a detector detects light emitted by the detectable molecules. A hardware processor determines a location of each capture structure on the sample plate surface, reads the capture structure code of each capture structure, and determines a location at which a target analyte is bound to one of the capture structures.
The method includes creating the plurality of capture structures, attaching each capture structure to a sample plate surface, and mixing the plurality of capture structures with the sample. The method further determines the location of each capture structure, reads the capture structure code of each capture structure, determines the location at which a target analyte is bound, and determines the target analyte based on the capture structure code at that bound location.
Claims Coverage
Two independent claims are present, centered on capture-structure codes embodied by nucleic-acid or polymer flowers with detectable molecules, and on reading those codes to identify target analytes at bound locations. The claims include three inventive features shared across the system and method.
Capture structures with code-embodying nucleic-acid or polymer flowers
A plurality of capture structures attached to the sample plate surface, each comprising a detection portion configured to couple with a target analyte and a stem comprising a capture structure code, the capture structure code comprising a plurality of flowers each independently comprising a piece of nucleic acid or polymer coupled to at least one detectable molecule, wherein the detectable molecules embody the capture structure code and in combination uniquely identify a type of the capture structure associated with the detection portion.
System to locate, read, and map analyte binding to code-defined capture locations
An excitation source configured to generate excitation energy delivered into the sample plate, a detector configured to detect light emitted by the detectable molecules, and a hardware processor configured to determine a location of each capture structure on the sample plate surface, read the capture structure code of each capture structure, determine a location at which a target analyte is bound to one of the capture structures, and determine the target analyte based on the capture structure code of the capture structure at the location where the target analyte is bound.
Method using code-defined capture structures to determine target analyte at bound locations
Creating a plurality of capture structures, each comprising a detection portion and a stem with a capture structure code comprising a plurality of flowers with nucleic acid or polymer coupled to detectable molecules embodying the capture structure code, attaching each capture structure to a sample plate surface, mixing the plurality of capture structures with the sample, determining a location of each capture structure, reading the capture structure code of each capture structure, determining a location at which a target analyte is bound to one of the capture structures, and determining the target analyte based on the capture structure code of the capture structure at the location at which the target analyte is bound.
The inventive subject matter is the use of capture-structure codes embodied by detectable molecules on nucleic-acid or polymer flowers to identify capture structure types and determine target analytes from the bound capture-structure locations.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
Interested in licensing this patent?