System and method for isolating extracellular vesicles

Inventors

Katakowski, Mark • Hozeska-Solgot, Ann

Assignees

Supershot Inc

Abstract

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

Core Innovation

The invention provides a system for isolating extracellular vesicles using a concentration system that processes blood or bone marrow. The system includes a first input port for receiving one or more of blood or bone marrow and a centrifuge device coupled to the first input port for separating fractions of one or more of red blood cells, platelet poor plasma, or platelet rich plasma.

The system further includes a first receptacle having one or more of platelet rich plasma fractions or platelet poor plasma fractions centrifuged from the centrifuge device. An aqueous two-phase solution is received into the first receptacle via a second input port coupled to the first receptacle, where the aqueous two-phase solution is delivered via a syringe coupled to the second input port.

After the centrifuge device separates one or more of the blood and the bone marrow into red blood cells, platelet poor plasma, or platelet rich plasma fractions, the aqueous two-phase solution is added to the platelet rich plasma fractions or platelet poor plasma fractions disposed in the first receptacle. The aqueous two-phase solution and the one or more of the platelet rich plasma fractions or platelet poor plasma fractions are drawn back into the centrifuge device for centrifugation to isolate extracellular vesicles and to create a pellet for injection, the pellet comprising the extracellular vesicles.

Claims Coverage

The independent claim covers a centrifuge-based workflow that separates blood or bone marrow into red blood cells, platelet poor plasma, and/or platelet rich plasma fractions, adds an aqueous two-phase solution to the platelet fractions in a receptacle via a syringe, then draws the mixture back into the centrifuge device to isolate extracellular vesicles and form an injection pellet. The inventive features are further refined by dependent claims specifying syringe delivery, PEG-DEX, volume determination, and downstream isolation using an isolation syringe and outlet port.

Across the independent claim and its dependent refinements, the system isolates extracellular vesicles from platelet poor plasma and/or from platelet rich plasma fractions by adding a syringe-delivered aqueous two-phase solution, including concentrated PEG-DEX, to platelet fractions in a receptacle, drawing the mixture back into the centrifuge device for centrifugation, and creating an injection pellet comprising extracellular vesicles, with downstream collection using an isolation syringe and an outlet port.

Stated Advantages

Documented Applications