Genome extraction device including flow cover
Inventors
Cho, Young-Shik • Lee, Sunyoung • LIM, Kwanhun • KIM, In-Ae • Kim, Dong-hun • Park, Hae-Joon • PARK, Hyo-Lim
Assignees
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Publication Number
US-12521711-B2
Publication Date
2026-01-13
Expiration Date
Abstract
Provided is a genome extraction device including a flow cover, more particularly a genome extraction device which includes a base plate, thereby forming a closed flow path inside and preventing a problem of narrowing of the flow path according to close contact coupling between components.
Core Innovation
The invention is a genome extraction device having an outer chamber partitioned into a plurality of first spaces by an outer chamber partition wall, where different reagents are accommodated in the plurality of first spaces. The device further includes a base plate coupled to a lower portion of the outer chamber and having a plurality of flow paths communicating with the plurality of first spaces, wherein the plurality of flow paths are formed on an upper surface of the base plate. A flow cover is disposed between the outer chamber and the base plate, and flow cover holes connecting the plurality of first spaces and the plurality of flow paths are formed through the flow cover.
A pad is disposed on an upper portion of the flow cover, and pad holes are formed through the pad to connect the flow cover holes and the plurality of first spaces. The device includes a piston installed in the outer chamber with a fluid accommodating portion formed in the piston, communicating with any one of the plurality of flow paths by rotation, and a driving unit coupled to the piston to rotate the piston. A geometric arrangement aligns the one end of the plurality of flow paths, the first flow cover holes, and the first pad holes, and aligns the other end of some flow paths, the second flow cover holes, and the second pad holes.
The invention defines a radial flow-path layout in which the plurality of flow paths extend outwardly in a radial direction from a portion spaced by a predetermined distance from a center of the base plate, and includes first and second circumferences at first and second distances from the center. Protrusions are formed on an upper surface of the pad in a portion where the second pad holes are formed, such that the diameter of the second pad holes is not reduced differently than intended. The flow cover is plastic material and the pad is elastic material, and the pad is disposed and compressed between the outer chamber and the base plate.
The flow cover and pad hole regions and the molded flow-path layout support preventing micro-leakage and cross-contamination, including dual-chamber spacing and a curved lower inner chamber configured to inhibit capillary action, and closed flow paths for protecting moisture-sensitive beads via dehumidification. An amplification module interface is described with separate gas and extract passages and differentiation in passage thickness/length to reduce mixing, and a dual-structured bead chamber includes a dehumidification chamber and dehumidification units to limit moisture ingress.
Claims Coverage
Independent claim clm-00001 defines the core genome extraction device with a multi-space outer chamber, a base plate with radial flow paths, a flow cover and pad with aligned holes, and a rotating piston fluid accommodating portion driven by a driving unit; it contains multiple structural inventive features expressed as geometric alignments, hole/circumference constraints, and specific sealing/assembly relationships.
Outer chamber partitioned reagent spaces
An outer chamber partitioned into a plurality of first spaces by an outer chamber partition wall and in which different reagents are accommodated in the plurality of first spaces.
Base plate with radial flow paths communicating with first spaces
A base plate coupled to a lower portion of the outer chamber and having a plurality of flow paths communicating with the plurality of first spaces, wherein the plurality of flow paths are formed on an upper surface of the base plate.
Flow cover between outer chamber and base plate with connecting flow cover holes
A flow cover disposed between the outer chamber and the base plate and through which flow cover holes connecting the plurality of first spaces and the plurality of flow paths are formed.
Pad on flow cover with pad holes connecting spaces and flow cover holes
A pad disposed on an upper portion of the flow cover and through which pad holes connecting the flow cover holes and the plurality of first spaces are formed.
Rotatable piston with fluid accommodating portion communicating with flow paths
A piston installed in the outer chamber, wherein a fluid accommodating portion is formed in the piston and communicates with any one of the plurality of flow paths by rotation, and a driving unit coupled to the piston to rotate the piston.
Radial multi-circumference flow-path and hole alignment
The plurality of flow paths extend outwardly in a radial direction from a portion spaced by a predetermined distance from a center of the base plate, with first and second circumferences at first and second distances, wherein first flow cover holes and first pad holes are disposed on the first circumference and second flow cover holes and second pad holes are disposed on the second circumference, and wherein alignment is provided between the ends of the plurality of flow paths, flow cover holes, and pad holes on each circumference.
Pad protrusions preventing unintended reduction of second pad-hole diameter
Protrusions that protrude from a portion in which the second pad holes are formed and narrow toward an upper side are formed on an upper surface of the pad such that the diameter of the second pad holes is not reduced differently than intended.
Plastic flow cover and elastic pad compressed between outer chamber and base plate
The flow cover is formed of a plastic material and the pad is formed of an elastic material, wherein the pad is disposed and compressed between the outer chamber and the base plate.
Coupling protrusion and driving unit insertion hole through-hole assembly
A first through hole in which the driving unit is installed is formed in the flow cover, a first coupling protrusion protruding upward and downward is formed on an outer circumference of the first through hole, and the first coupling protrusion is inserted into a driving unit insertion hole of the base plate and a second through hole of the pad, with the piston including a portion penetrating the first through hole and the second through hole while contacting inner/outer circumferential surfaces of the coupling protrusion and the second through hole.
Overall, claim clm-00001 covers a genome extraction device where reagent-containing outer chamber spaces communicate through a precisely aligned flow cover and elastic pad with radial base-plate flow paths, and a rotating piston with a fluid accommodating portion selects communicated flow paths via a driving unit, with additional geometric and protrusion constraints to control pad-hole diameter and with a plastic/elastic compressed, coupling-protrusion assembly.
Stated Advantages
Prevents micro-leakage and cross-contamination.
Inhibits capillary action via dual-chamber spacing and a curved lower inner chamber.
Reduces mixing in an amplification module by providing separate gas and extract passages.
Protects moisture-sensitive beads by forming closed flow paths and using dehumidification to minimize ambient moisture ingress.
Ensures the diameter of second pad holes is not reduced differently than intended.
Documented Applications
Genome extraction using a genome extraction device architecture including an amplification module interface and a bead chamber with dehumidification.
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