Nanocomposites and nanoagents for detection and treatment of a target of interest and methods of making and using same
Inventors
Biris, Alexandru S. • Nima, Zeid
Assignees
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Publication Number
US-12467920-B2
Publication Date
2025-11-11
Expiration Date
Abstract
A nanoagent for detections and treatments of multiple targets of interest includes multiple types of nanocomposites, each type of nanocomposites comprising at least one nanostructure, each nanostructure having a core and a shell surrounding the core; a respective reporter assembled on the shell of each nanostructure; and a layer of a respective treating agent and a respective targeting agent conjugated to the respective reporter. In use, each type of nanocomposite targets to a respective target of interest according to the respective targeting agent and releases the respective treating agent and the nanostructure therein for therapeutic treatment of the respective target of interest, and the respective target of interest transmits at least one signature responsive to the respective reporter for detection of the respective target of interest.
Core Innovation
The disclosed invention provides a method of making a nanocomposite for detection and treatment of a target of interest, wherein the target of interest comprises tumor cells or pathogens. Each nanocomposite is formed from at least one nanostructure having a core and a shell surrounding the core, and a reporter is assembled on the shell for respectively transmitting at least one signature from the target of interest.
The method further includes conjugating a layer of a treating agent and/or a targeting agent to the reporter. The treating agent is adapted for treating the target of interest, and the targeting agent is adapted for targeting the nanocomposite to the target of interest. The reporter comprises 4-mercaptobenzoic acid (4MBA), p-aminothiophenol (PATP), p-nitrothiophenol (PNTP), 4-(methylsulfanyl) thiophenol (4MSTP), molecules with a unique Raman spectral signature, or a fluorescent agent.
Dependent refinements include adding a thiolated PEG layer and specifying a conjugation workflow using PBS with NHS and EDC. Another refinement specifies that each core includes a gold nanorod and the shell includes a layer of silver nanoparticles.
Claims Coverage
The independent claim covers a method of making a nanocomposite for detection and treatment of tumor cells or pathogens. It includes three main inventive features: core-shell nanostructure formation, reporter assembly on the shell to transmit a target signature, and conjugation of treating and/or targeting agents to the reporter, with specific reporter chemistries or a fluorescent agent.
Core and shell nanostructure formation for target detection and treatment
Forming at least one nanostructure, each nanostructure having a core and a shell surrounding the core, for a target of interest comprising tumor cells or pathogens.
Shell-mounted reporter transmitting target signature
Assembling a reporter on the shell of each nanostructure, wherein the reporter is adapted for respectively transmitting at least one signature from the target of interest, and wherein the reporter comprises 4MBA, PATP, PNTP, 4MSTP, molecules with a unique Raman spectral signature, or a fluorescent agent.
Conjugation of treating and/or targeting agents to the reporter
Conjugating a layer of a treating agent and/or a targeting agent to the reporter, wherein the treating agent is adapted for treating the target of interest, and the targeting agent is adapted for targeting the nanocomposite to the target of interest.
The claims center on making a core-shell nanostructure nanocomposite in which a shell-mounted reporter conveys a target-associated signature and is linked to treating and/or targeting agents for detecting and treating tumor cells or pathogens, with dependent refinements specifying thiolated PEG coating, PBS/NHS/EDC conjugation workflow, and gold nanorod cores with silver nanoparticle shells.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Detection and treatment of a target of interest comprising tumor cells or pathogens using the disclosed nanocomposite.
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