Methods and compositions to select and/or predict cotton plants resistant to Fusarium race-4 resistance in cotton

Inventors

Buyyarapu, Ramesh • Parliament, Kelly • MAHILL, Joel F • Anderson, Timothy J.

Assignees

Corteva Agriscience LLC

Abstract

This disclosure concerns methods and compositions for identifying cotton plants that have a Fusarium wilt Race 4 resistance trait. Some embodiments concern molecular markers to identify, select, and/or construct Fusarium wilt Race 4 resistant plants and germplasm, or to identify and counter select relatively susceptible plants. This disclosure also concerns cotton plants comprising a Fusarium wilt Race 4 resistance trait that are generated by methods utilizing at least one marker described herein.

Core Innovation

The disclosure relates to identifying, selecting, and introgressing Fusarium wilt Race 4 (FOV-R4) resistance in Gossypium using molecular markers tightly linked to a major FOV-R4 QTL region on cotton chromosome 17. Marker/probe sets detect FOV-R4-resistant versus susceptible plants or germplasm by using SNP marker alleles within defined physical intervals, and marker detection supports marker-assisted selection (MAS) and marker-assisted backcrossing.

The disclosure describes screening nucleic acid samples from one or more Gossypium plants or germplasm for specific FOV-R4 resistance marker alleles, including alleles identified by position information relative to SEQ ID NOs. A first Gossypium plant or germplasm identified as having the screened resistance marker allele is selected, and progeny plants are produced by crossing with a second Gossypium plant such that at least one progeny plant comprises the FOV-R4 resistance marker allele.

The disclosure also provides approaches for marker detection and allele correlations with resistance, and includes a polyploid SNP discovery/validation pipeline (HAPSNP) together with SNP chip genotyping and assay conversion. The marker-based approaches are extended to introgress markers from a donor into a recipient cotton, and the disclosure describes automated systems and kits for marker detection.

Claims Coverage

The independent claims cover a method for producing a Gossypium plant having Fusarium wilt Race 4 (FOV-R4) resistance by nucleic-acid sampling, screening for a particular resistance marker allele defined by allele identity and sequence position, selecting plants or germplasm carrying that allele, and crossing to generate progeny that carry the allele. The inventive features focus on marker-allele screening tied to a specific SEQ ID position and the resulting marker-defined resistance in progeny.

The claim coverage centers on producing FOV-R4 resistant Gossypium plants by screening for a specific resistance marker allele (allele C at position 199 of SEQ ID NO: 83), selecting plants or germplasm that carry that allele, and crossing to generate progeny that retain the marker allele. A refinement specifies that the marker allele is from a Gossypium barbadense line.

Stated Advantages

Documented Applications