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Publication Number
US-12448408-B2
Publication Date
2025-10-21
Expiration Date
Abstract
A method for producing a capped RNA which is an RNA having the 5′-end modified with a cap, the method including: reacting an activated capping compound represented bywith a monophosphate RNA having the 5′-end monophosphorylated, where, L represents a leaving group. The activated capping compound is preferably a compound represented by
Core Innovation
The disclosed subject matter relates to a chemical method for producing a capped RNA, which is an RNA having a 5′-end modified with a cap. The method reacts a 5′-monophosphate RNA with an activated capping compound represented by Chemical Formula 1, where the activated capping compound is a diphosphate compound having a leaving group L.
The chemical cap introduction is performed without enzymes by using the activated capping compound together with a heteroaromatic imidazole derivative and a metal salt in a solvent. The disclosed embodiments emphasize selection of imidazole derivatives, including N-alkylimidazole and 2-nitroimidazole, and metal salts.
The document further provides that reaction temperature, the metal salt, the type and concentration of the imidazole additive, and solvent water content affect cap introduction efficiency. Preferred reaction conditions include a reaction temperature range and reaction time, and an organic solvent with limited water is selected.
Claims Coverage
The independent claim covers producing a capped RNA by reacting an activated capping compound with a 5′-monophosphate RNA in the presence of a heteroaromatic compound, a metal salt, and a solvent; four inventive features are identified.
Producing a capped RNA via Chemical Formula 1 activated capping compound
Reacting an activated capping compound represented by Chemical Formula 1 with a 5′-monophosphate RNA to produce a capped RNA whose 5′ end is modified with a cap.
Using a heteroaromatic compound in the capping reaction
Performing the reacting in the presence of a heteroaromatic compound.
Using a metal salt and a solvent
Performing the reacting in the presence of a metal salt and a solvent.
Leaving group L on the activated capping compound
Employing the activated capping compound where L represents a leaving group.
Claim coverage centers on chemical production of a 5′-capped RNA by reacting a Chemical Formula 1 activated capping compound with a 5′-monophosphate RNA, where the reaction uses a heteroaromatic compound, a metal salt, and a solvent, and the activated capping compound includes a leaving group L.
Stated Advantages
Simple chemical cap introduction without enzymes.
Documented Applications
Producing a capped RNA in which the RNA has a 5′-end modified with a cap.
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