Loop-mediated isothermal amplification devices and systems for detecting genetic material
Inventors
Kalkum, Markus • Roeth, Daniel • Bishara, Isaac • Rusnak, Miro • DADWAL, Sanjeet
Assignees
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Publication Number
US-12440844-B2
Publication Date
2025-10-14
Expiration Date
Abstract
Provided are methods, devices, and systems for a loop-mediated isothermal amplification (LAMP) reactions for detecting the presence of genetic material. A LAMP device includes an enclosure having outer walls configured to define an internal space. The LAMP device also includes a tray assembly configured to support a reaction vessel configured for holding a sample for amplification. The LAMP device also includes a plurality of light emitters configured to illuminate the reaction vessel for exciting a fluorescence of the sample in the reaction vessel. The LAMP device also includes an imaging device configured to obtain an image of the sample for determining a quantity of the fluorescence in the sample. The LAMP device also includes a controller configured to regulate a temperature of the tray assembly and instruct the imaging device to obtain the image for determining the quantity of the fluorescence in the sample.
Core Innovation
The invention provides a portable device configured to conduct loop-mediated isothermal amplification (LAMP) inside an enclosure. The enclosure has outer walls configured to define an internal space and isolates the internal space from light outside the enclosure. A tray assembly is situated within the internal space to support a reaction vessel for holding a sample for amplification.
The device includes a plurality of light emitters situated inside the enclosure to illuminate the reaction vessel and excite fluorescence of the sample in the reaction vessel. An imaging device is situated in the internal space and obtains an image of the sample for determining a quantity of the fluorescence.
A controller communicatively coupled to the tray assembly, the plurality of light emitters, and the imaging device regulates a temperature of the tray assembly and instructs the imaging device to obtain the image for determining the quantity of fluorescence. The disclosure further indicates determination of presence of pathogenic material from the fluorescence, including quantification using fluorescence signal analysis such as fitting to amplification curves and RFU thresholding.
Claims Coverage
Independent claim clm-00001 covers a LAMP device architecture that combines a light-isolated enclosure, an internal tray assembly holding a reaction vessel, internal fluorescence excitation with light emitters, imaging to quantify fluorescence, and controller-based temperature regulation with imaging instruction. It includes five principal inventive areas: enclosure light isolation, tray and vessel arrangement, internal fluorescence illumination, imaging-based fluorescence quantity determination, and controller-regulated temperature with image acquisition.
Light-isolated enclosure for internal space
An enclosure having outer walls configured to define an internal space, the enclosure configured to isolate the internal space from light outside the enclosure.
Tray assembly for supporting a reaction vessel
A tray assembly situated within the internal space, configured to support a reaction vessel for holding a sample for amplification.
Internal light emitters to excite fluorescence
A plurality of light emitters situated inside the enclosure, configured to illuminate the reaction vessel for exciting fluorescence of the sample in the reaction vessel.
Imaging device to determine fluorescence quantity
An imaging device situated in the internal space and configured to obtain an image of the sample for determining a quantity of the fluorescence in the sample.
Controller-regulated tray temperature and imaging instruction
A controller communicatively coupled to the tray assembly, the plurality of light emitters, and the imaging device, configured to regulate a temperature of the tray assembly and instruct the imaging device to obtain the image for determining the quantity of the fluorescence in the sample.
Overall, the claim set centers on conducting LAMP in an enclosure that isolates internal optical measurements, using internal illumination to excite fluorescence in a reaction vessel, quantifying fluorescence via imaging, and coordinating temperature regulation with image acquisition using a controller. Dependent claims refine structural placement and add interpretation of fluorescence for determining pathogenic material presence.
Stated Advantages
Enables determination of a quantity of fluorescence in a sample using internal illumination and an imaging device.
Enables determination of presence of pathogenic material from the fluorescence signal.
Documented Applications
Saliva-to-LAMP detection for SARS-CoV-2, with genomic RNA isolation and fluorescence-based identification of pathogenic material indicated in the summarized disclosure.
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