Affinity membrane and method of preparation
Inventors
ZHOU, JINXIANG • Temples, Graham • Henn, Daniel
Assignees
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Publication Number
US-12434201-B2
Publication Date
2025-10-07
Expiration Date
Abstract
A method for preparing an adsorptive media for binding biologic molecules comprising immersing a macroporous support in a first solution of a coupling reagent in a solvent solution for attachment of said coupling reagent to form coupling groups; and, immersing said macroporous support in an incubating solution selected from the group consisting of ligand, nucleotide, oligonucleotide, peptide, polypeptide, protein, and enzyme solutions having an affinity to a biologic target molecule to couple one of said ligands, nucleotides, oligonucleotides, peptides, polypeptides, proteins, and enzymes to at least a portion of said coupling groups of said macroporous support for binding with said biologic target molecule when exposed to said macroporous support.
Core Innovation
The disclosed invention relates to affinity membrane media in which protein A is covalently bonded to the surface of a macroporous support. The macroporous support includes cellulose-based materials such as cellulose ester membranes, cellulose acetate membranes, regenerated cellulose membranes, cellulosic nanofiber membranes, cellulosic monoliths, filter paper membranes, cellulose, derivatives of cellulose, and combinations thereof.
The media are characterized by affinity binding performance toward human immunoglobulin G, including dynamic binding capacity at 10% breakthrough with very short residence time. The described media exhibit dynamic binding capacity at 10% breakthrough of at least 20 mg human immunoglobulin G per milliliter of macroporous support at a residence time of 6 seconds or less.
In related forms, the media are characterized by a static binding capacity greater than 60 mg human immunoglobulin G per milliliter of macroporous support. The disclosure also specifies macroporous support configurations and surface features, including pores with a pore size range, stacks with specified thickness, and adsorptive groups comprising a tertiary amine on the support surface.
Claims Coverage
The partial record contains two independent claims. Across these claims, the inventive subject matter centers on protein A covalently bonded to a macroporous cellulose-based support and evaluated by static and/or dynamic IgG binding capacity metrics.
Protein A covalently bonded to a macroporous cellulose-based support for high dynamic IgG binding
A media comprising a macroporous support comprising protein A covalently bonded to a surface of the macroporous support, wherein the media exhibit a dynamic binding capacity at 10% breakthrough of at least 20 mg of human immunoglobulin G per milliliter of the macroporous support at a residence time of 6 seconds or less, and the macroporous support comprises a cellulose ester membrane, a cellulose acetate membrane, a regenerated cellulose membrane, a cellulosic nanofiber membrane, a cellulosic monolith, a filter paper membrane, cellulose, a derivative of cellulose, or combinations thereof.
Protein A covalently coupled to a macroporous cellulose-based support for high static IgG binding
A media comprising a macroporous support comprising protein A covalently coupled to a surface of the macroporous support, wherein the adsorptive media exhibit a static binding capacity of greater than 60 mg human immunoglobulin G per milliliter of the macroporous support, and the macroporous support comprises a cellulose ester membrane, a cellulose acetate membrane, a regenerated cellulose membrane, a cellulosic nanofiber membrane, a cellulosic monolith, a filter paper membrane, cellulose, a derivative of cellulose, and combinations thereof.
Overall claim coverage is directed to affinity media built from a macroporous cellulose-based support having protein A covalently bonded or coupled to its surface, with binding performance quantified as either dynamic binding capacity at 10% breakthrough at residence times of 6 seconds or less, or static binding capacity greater than 60 mg human immunoglobulin G per milliliter of support.
Stated Advantages
Exhibiting a dynamic binding capacity at 10% breakthrough of at least 20 mg human immunoglobulin G per milliliter of macroporous support at a residence time of 6 seconds or less.
Exhibiting a static binding capacity greater than 60 mg human immunoglobulin G per milliliter of macroporous support.
Documented Applications
Not explicitly described in patent.
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